HUMAN MAST-CELLS RECOVERED BY BRONCHOALVEOLAR LAVAGE - THEIR MORPHOLOGY, HISTAMINE-RELEASE AND THE EFFECTS OF SODIUM CROMOGLYCATE
HUMAN MAST-CELLS RECOVERED BY BRONCHOALVEOLAR LAVAGE - THEIR MORPHOLOGY, HISTAMINE-RELEASE AND THE EFFECTS OF SODIUM CROMOGLYCATE
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DOI：
10.1042/cs0680427
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发表时间：
1985-01-01
期刊：
影响因子：
6
通讯作者：
JOHNSON, NM
中科院分区：
文献类型：
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作者：
FLINT, KC;LEUNG, KBP;JOHNSON, NM
肥大细胞占支气管肺泡灌洗（BAL）恢复细胞总数的0.5 - 3%（平均1.35%）。这些细胞大多数具有粘膜肥大细胞的形态学特征，在甲醛盐水中固定后，在阿利新蓝-番红花苷反应中不能染色，而在卡诺伊溶液中固定后染色良好。用硫酸小檗碱染色的细胞仅在26次灌洗中4次可见。BAL细胞对抗人免疫球蛋白E （IgE）反应释放组胺，呈剂量依赖性，抗IgE稀释比例为1:100时效果最佳。最大释放时间为2 min。2-脱氧葡萄糖（5 mmol/l）和抗霉素a （1 mmol/l）联合使用可完全抑制组胺的释放。甘露糖酸二钠（DSCG）在1 mmol/l （P < 0.02）、100 mmol/l （P < 0.002）和10 mmol/l （P < 0.003）浓度下显著抑制组胺释放，10 mmol/l浓度下最大抑制率为50.1%。
Mast cells make up between 0.5 and 3%(mean 1.35%) of total cells recovered by bronchoalveolar lavage (BAL). The majority of these cells have the morphological characteristics of mucosal mast cells in that they fail to stain in the alcian blue-safranin reaction after fixation in formol-saline but stain well after fixation in Carnoy's solution. Cells staining with berberine sulphate were seen in only four of the 26 lavages. BAL cells released histamine in response to anti-human immunoglobulin E (IgE) in a dose-dependent manner that was optimal at a dilution of anti-IgE of 1: 100. Maximum release was obtained by 2 min. Histamine release was completely inhibited by a combination of 2-deoxyglucose (5 mmol/l) and antimycin A (1 mumol/l). Disodium cromoglycate (DSCG) significantly inhibited this histamine release at 1 mmol/l (P less than 0.02), 100 mumol/l (P less than 0.002) and 10 mumol/l (P less than 0.003), with maximum inhibition of 50.1% at 10 mumol/l.