Soft X-ray microscopy with a cryo scanning transmission X-ray microscope: I. Instrumentation, imaging and spectroscopy
Soft X-ray microscopy with a cryo scanning transmission X-ray microscope: I. Instrumentation, imaging and spectroscopy
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DOI：
10.1046/j.1365-2818.2000.00630.x
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发表时间：
2000-01-01
期刊：
影响因子：
--
通讯作者：
Tennant, D
中科院分区：
文献类型：
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作者：
Maser, J;Osanna, A;Tennant, D
我们研制了一种低温扫描透射X射线显微镜，它使用国家同步辐射光源的软X射线。该系统能够在100 K左右的温度下对厚度达10 μ m的冷冻水合样品进行成像。我们展示了冷冻水合真核细胞的图像和光谱，并证明生物标本在辐射剂量高达约10（10）格雷时不会发生质量损失或形态变化。这使得需要相同样本区域的多个图像的研究成为可能，例如断层扫描（Wang等人（2000）Soft X-ray microscopy with a cryo scanning transmission X-ray microscope：II.断层扫描。J. Microsc. 197，80-93）或光谱分析。
We have developed a cryo scanning transmission X-ray microscope which uses soft X-rays from the National Synchrotron Light Source. The system is capable of imaging frozen hydrated specimens with a thickness of up to 10 mu m at temperatures of around 100 K. We show images and spectra from frozen hydrated eukaryotic cells, and a demonstration that biological specimens do not suffer mass loss or morphological changes at radiation doses up to about 10(10) Gray. This makes possible studies where multiple images of the same specimen area are needed, such as tomography (Wang et al. (2000) Soft X-ray microscopy with a cryo scanning transmission X-ray microscope: II. Tomography. J. Microsc. 197, 80-93) or spectroscopic analysis.