HEALING OF PARTIAL THICKNESS PORCINE SKIN WOUNDS IN A LIQUID ENVIRONMENT
HEALING OF PARTIAL THICKNESS PORCINE SKIN WOUNDS IN A LIQUID ENVIRONMENT
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DOI：
10.1016/0022-4804(92)90278-8
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发表时间：
1992-01-01
影响因子：
2.2
通讯作者：
MILLER, DR
中科院分区：
文献类型：
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作者：
BREUING, K;ERIKSSON, E;MILLER, DR
本研究采用不透液的乙烯基室对猪的实验性伤口进行局部液相治疗。生理盐水持续治疗可明显减少二度烧伤早期组织破坏的进展，未覆盖的烧伤形成较深的坏死层（0.49 ± 0.004 mm，平均值± SD），而覆盖有空腔的烧伤创面坏死较少（0.14 ± 0.01 mm），液体治疗的创面不形成焦痂，可检测到很少的组织坏死（<0.005 mm）。在创伤后的前5天内，与盐水处理的伤口相比，用高渗葡聚糖局部治疗使穿过烧伤伤口的水通量增加了0.24 ml/cm 2/24 hr（平均值，n= 95）。当将部分厚度烧伤和切除伤口浸入等渗盐水中直至愈合时，伤口表面的水、蛋白质、电解质和葡萄糖的每日流出量在愈合期间下降至对照组（盐水覆盖的未受伤皮肤）中发现的基线值。下降的蛋白质渗透性被用作上皮屏障功能恢复的可重复的、非侵入性的内源性标志物。生理盐水处理的切除伤口在8.6 ± 0.6天内愈合（平均值± SD，n= 27），烧伤伤口在12.1 ± 1.4天内愈合（平均值± SD，n = 15）。液体处理伤口的愈合没有组织浸渍，并且比空气暴露伤口的愈合显示出更少的炎症和更少的瘢痕形成（没有尝试比较空气和液体暴露伤口之间的愈合率）。我们认为充满液体的腔室是一个潜在的非常有用的诊断，监测和输送系统，用于伤口愈合研究和人类伤口治疗。
This study employs a liquid-tight vinyl chamber for the topical fluid-phase treatment of experimental wounds in pigs. Continuous treatment with normal saline significantly reduced the early progression of tissue destruction in partial thickness burns.Uncoveredburns formed a deep layer of necrosis (0.49 ± 0.004 mm, mean ± SD) although burn wounds covered withempty chambersdemonstrated less necrosis (0.14 ± 0.01 mm),fluid-treatedwounds formed no eschar, and little tissue necrosis could be detected (<0.005 mm). Topical treatment with hypertonic dextran increased water flux across burn wounds by 0.24 ml/cm2/24 hr (mean,n= 95) over saline-treated wounds during the first 5 days after wounding. When partial thickness burn and excisional wounds were immersed in isotonic saline until healed, the daily efflux of water, protein, electrolytes, and glucose across the wound surface declined during healing to baseline values found in controls (saline-covered unwounded skin). The declining protein permeability was used as a reproducible, noninvasive, endogenous marker for the return of epithelial barrier function. Saline-treated excisional wounds healed within 8.6 ± 0.6 days (mean ± SD,n= 27) and burn wounds within 12.1 ± 1.4 days (mean ± SD, n = 15). Healing of fluid-treated wounds occurred without tissue maceration and showed less inflammation and less scar formation than healing of air exposed wounds (no attempt was made to compareratesof healing between air- and fluid-exposed wounds). We consider the fluid-filled chamber a potentially very useful diagnostic, monitoring, and delivery system for wound-healing research and for human wound therapy.