Exposure of rat brain to 915 MHz GSM microwaves induces changes in gene expression but not double stranded DNA breaks or effects on chromatin conformation
Exposure of rat brain to 915 MHz GSM microwaves induces changes in gene expression but not double stranded DNA breaks or effects on chromatin conformation
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DOI：
10.1002/bem.20216
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发表时间：
2006-05-01
影响因子：
1.9
通讯作者：
Persson, BRR
中科院分区：
文献类型：
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作者：
Belyaev, IY;Koch, CB;Persson, BRR
我们研究了暴露于全球移动的通信系统（GSM）的微波（MW）是否会诱导DNA断裂，染色质构象和基因表达的变化。基于采用915 MHz GSM信号的测试移动的电话使用曝光装置，包括所有标准调制，脉冲输出功率水平为2 W，比吸收率（SAR）为0.4 mW/g。将大鼠暴露于或假暴露于MW 2 h。暴露后，从脑样品以及脾脏和胸腺制备细胞悬液。为了分析基因表达模式，从小脑提取总RNA。染色质构象的变化，这是应激反应和遗传毒性效应的指示，测量异常粘度时间依赖性（AVTD）的方法。DNA双链断裂（DSB）通过脉冲场凝胶电泳（PFGE）进行分析。MW暴露对染色质构象和DNA双链断裂均无影响。通过代表8800个大鼠基因的Affyssin U34 GeneChips获得基因表达谱，并用Affyssin Microarray Suite（MAS）5.0软件进行分析。在所有暴露动物的小脑中，I I基因表达上调1.34-2.74倍，一个基因表达下调0.48倍（P
We investigated whether exposure of rat brain to microwaves (MWs) of global system for mobile communication (GSM) induces DNA breaks, changes in chromatin conformation and in gene expression. An exposure installation was used based on a test mobile phone employing a GSM signal at 915 MHz, all standard modulations included, output power level in pulses 2 W, specific absorption rate (SAR) 0.4 mW/g. Rats were exposed or sham exposed to MWs during 2 h. After exposure, cell suspensions were prepared from brain samples, as well as from spleen and thymus. For analysis of gene expression patterns, total RNA was extracted from cerebellum. Changes in chromatin conformation, which are indicative of stress response and genotoxic effects, were measured by the method of anomalous viscosity time dependencies (AVTD). DNA double strand breaks (DSBs) were analyzed by pulsed-field gel electrophoresis (PFGE). Effects of MW exposure were observed on neither conformation of chromatin nor DNA DSBs. Gene expression profiles were obtained by Affymetrix U34 GeneChips representing 8800 rat genes and analyzed with the Affymetrix Microarray Suite (MAS) 5.0 software. In cerebellum from all exposed animals, I I genes were upregulated in a range of 1.34-2.74 fold and one gene was downregulated 0.48-fold (P