Col1a1-GFP transgene expression in developing incisors
Col1a1-GFP transgene expression in developing incisors
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DOI：
10.1080/03008200290001078
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发表时间：
2002-04-01
影响因子：
2.9
通讯作者：
Mina, M
中科院分区：
文献类型：
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作者：
Braut, A;Kalajzic, I;Mina, M
以往的研究表明，终末分化的成牙本质细胞是伴随着显着增加I型胶原的合成。最近产生了转基因小鼠，其中绿色荧光蛋白（GFP）表达受大鼠3.6（pOBCol3.6GFPtpz）和2.3（pOBCol2.3GFPemd）Col 1a 1启动子片段的控制。我们对这些表达GFP的转基因小鼠的分析表明，2.3-kb启动子片段仅指导GFP在骨骼和牙齿中的强表达，而3.6-kb启动子片段指导GFP在骨骼和牙齿以及其他I型胶原蛋白产生组织中的强表达。我们对这些转基因小鼠切牙的观察显示，在功能性成牙本质细胞和分化的成骨细胞中，GFP表达水平很高。这些观察结果表明，GFP报告基因的表达密切遵循α 1（I）胶原蛋白在各种组织，包括成牙本质细胞的表达模式。
Previous studies have shown that terminal differentiation of odontoblasts is accompanied by dramatic increases in type I collagen synthesis. Recently transgenic mice in which green fluorescent protein (GFP) expression is under the control of the rat 3.6 (pOBCol3.6GFPtpz) and 2.3 (pOBCol2.3GFPemd) Col1a1 promoter fragments were generated. Our analysis of these GFP-expressing transgenic mice shows that the 2.3-kb promoter fragment directs strong expression of GFP only to bones and teeth, whereas the 3.6-kb fragment of promoter directs strong expression of GFP in bone and tooth, as well as in other type I collagen producing tissues. Our observations of incisors in these transgenic mice show high levels of GFP expression in functional odontoblasts and in differentiated osteoblasts. These observations show that expression of GFP reporter genes closely follow the patterns of expression of alpha1(I) collagen in various tissues including odontoblasts.