Imaging Flow Cytometry for Phytoplankton Analysis: Instrumentation and Applications.
Imaging Flow Cytometry for Phytoplankton Analysis: Instrumentation and Applications.
复制标题
用于浮游植物分析的成像流式细胞术：仪器和应用。
DOI：
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发表时间：
2019
影响因子：
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通讯作者：
N. Poulton
中科院分区：
文献类型：
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作者：
N. Poulton
成像流式细胞术于20世纪90年代末首次引入藻类研究。从环境样本中枚举、分类和确定浮游植物生物量的能力对于确定生态系统功能、碳密度以及浮游植物在淡水和海洋微生物食物网中的作用至关重要。传统的微型浮游植物定量方法采用显微技术，需要保存，速度慢，繁琐，非常费力。自动成像显微镜平台的可用性已经彻底改变了在自然环境中检测粒子和细胞的方式。检测未经改变和保存的细胞的能力是提供更准确的细胞浓度估计和浮游植物生物量的关键。目前，许多成像细胞术工具可用于水生科学，并提供了一种更快速和公正的方法来枚举和分类不同水生环境中的浮游植物。成像流式细胞术将流式细胞术的速度和统计能力与显微镜的成像特性相结合，近年来对浮游植物分析的发展做出了重大贡献。本报告讨论了与浮游植物群落相关的各种仪器和应用。
Imaging flow cytometry was first introduced into algal research in the late 1990s. The ability to enumerate, classify and determine biomass of phytoplankton from environmental samples is essential for determining ecosystem function, carbon density, and the role phytoplankton play in both freshwater and marine microbial food webs. Traditional micro-phytoplankton quantification methods use microscopic techniques that require preservation and are slow, tedious and very laborious. The availability of automated imaging microscopy platforms has revolutionized the way particles and cells are detected within their natural environment. The ability to examine cells unaltered and without preservation is key to providing more accurate cell concentration estimates and phytoplankton biomass. Currently many imaging cytometry tools are available for use in aquatic sciences and provide a more rapid and unbiased method for enumerating and classifying phytoplankton within diverse aquatic environments. Imaging flow cytometry, combines the speed and statistical capabilities of flow cytometry with imaging features of microscopy, and has contributed significantly to the advancement of phytoplankton analysis in recent years. This presentation discusses various instrumentation and applications relevant tophytoplankton communities.