Bacteria of the genus Asaia stably associate with Anopheles stephensi, an Asian malarial mosquito vector
Bacteria of the genus Asaia stably associate with Anopheles stephensi, an Asian malarial mosquito vector
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DOI：
10.1073/pnas.0610451104
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发表时间：
2007-05-22
影响因子：
11.1
通讯作者：
Daffonchio, Daniele
中科院分区：
文献类型：
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作者：
Favia, Guido;Ricci, Irene;Daffonchio, Daniele
在这里，我们发现 Asaia 属的α-变形菌与史氏按蚊的幼虫和成虫稳定相关，史氏按蚊是间日疟原虫的重要蚊媒，间日疟原虫是亚洲的主要疟疾病原体。 16S rRNA 基因丰度、定量 PCR、透射电子显微镜和 165 rRNA 基因的原位杂交表明，Asaia 细菌在蚊子相关微生物群中占主导地位。在成年蚊子中，Asaia sp。在女性肠道和男性生殖道中以高密度存在。阿萨亚 sp。来自安. Stephensi 已在无细胞培养基中培养，然后用外源 DNA 进行转化。绿色荧光蛋白标记的 Asaia sp。菌株有效地滞留在女性肠道和唾液腺中，这些部位对疟原虫至关重要。的发展和传播。幼虫肠道和雄性生殖系统也被转化的 Asaia sp 定植。拉紧。作为传播疟原虫属 (Plasmodium sp.) 的蚊子的有效诱导定殖者，Asaia sp.。可能是控制疟疾的候选者。
Here, we show that an alpha-proteobacterium of the genus Asaia is stably associated with larvae and adults of Anopheles stephensi, an important mosquito vector of Plasmodium vivax, a main malaria agent in Asia. Asaia bacteria dominate mosquito-associated microbiota, as shown by 16S rRNA gene abundance, quantitative PCR, transmission electron microscopy and in situ-hybridization of 165 rRNA genes. In adult mosquitoes, Asaia sp. is present in high population density in the female gut and in the male reproductive tract. Asaia sp. from An. stephensi has been cultured in cell-free media and then transformed with foreign DNA. A green fluorescent protein-tagged Asaia sp. strain effectively lodged in the female gut and salivary glands, sites that are crucial for Plasmodium sp. development and transmission. The larval gut and the male reproductive system were also colonized by the transformed Asaia sp. strain. As an efficient inducible colonizer of mosquitoes that transmit Plasmodium sp., Asaia sp. may be a candidate for malaria control.