HOMEOVISCOUS ADAPTATION UNDER PRESSURE - THE PRESSURE-DEPENDENCE OF MEMBRANE ORDER IN BRAIN MYELIN MEMBRANES OF DEEP-SEA FISH
HOMEOVISCOUS ADAPTATION UNDER PRESSURE - THE PRESSURE-DEPENDENCE OF MEMBRANE ORDER IN BRAIN MYELIN MEMBRANES OF DEEP-SEA FISH
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DOI：
10.1016/0005-2736(92)90102-r
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发表时间：
1992-01-31
期刊：
影响因子：
--
通讯作者：
COSSINS, AR
中科院分区：
文献类型：
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作者：
BEHAN, MK;MACDONALD, AG;COSSINS, AR
1，6-二苯基-1，3，5-己三烯(DPH)荧光的稳态各向异性和时间分辨各向异性被用来比较浅水和两种深海鱼类(Coryhenoids rupestris和Coryhenoides armatus)脑髓鞘膜的碳氢化合物顺序。在大气压下，深海鱼类的稳态各向异性比浅水物种低，尽管所有物种的各向异性与压力的依赖关系相似。时间分辨测量允许从该运动的幅度中分离地确定探测器运动的速率。根据两个相关时间和一个常数(r(无穷大))来分析各向异性衰减。所有物种的r(无穷大)和有序参数都随着压力的增加而增加，深海物种的曲线图被转化为相对于浅水物种更高的压力。由此产生的华支睾吸虫的压力系数明显小于这两个较浅的物种。这些时间分辨的研究表明，当根据它们各自的栖息地压力和温度条件进行校正时，种间差异提供了所有三个物种相似的有序性参数。这表明，尽管高静水压对髓磷脂的有序性有深刻的影响，但髓磷脂的有序性是高度保守的。
Steady-state and time-resolved anisotropy of 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence have been used to compare the hydrocarbon order of brain myelin membranes from a shallow water (plaice) and two deep-sea fish species (Coryphenoides rupestris and Coryphenoides armatus). At atmospheric pressure the deep sea fish displayed lower steady-state anisotropies than shallow water species although the pressure dependence of anisotropy was similar in all species. Time-resolved measurements allowed the separate determination of the rate of probe motion from the amplitude of that motion. Anisotropy decays were analysed in terms of two correlation times and a constant (r(infinity)). The r(infinity) and order parameter for all species increased with pressure, the graphs for deep-sea species being translated to higher pressures relative to shallow-water species. The resulting pressure coefficients for C. armatus was distinctly less than for the two shallower species. These time-resolved studies show that the interspecific differences provide for similar order parameters in all three species when corrected to their respective habitat conditions of pressure and temperature. This indicates that myelin order is highly conserved despite the profound ordering effects of high hydrostatic pressure.