Three-dimensional molecular modeling with single molecule FRET.
Three-dimensional molecular modeling with single molecule FRET.
复制标题
DOI：
10.1016/j.jsb.2010.09.004
复制
发表时间：
2011-03
影响因子：
3
通讯作者：
Weninger, Keith R.
中科院分区：
文献类型：
--
作者：
Brunger, Axel T.;Strop, Pavel;Vrljic, Marija;Chu, Steven;Weninger, Keith R.
单分子荧光能量转移实验能够通过在大分子中的特定位点引入荧光染料来研究大分子的构象和折叠。可以用不同的标记位点组合进行多个这样的实验，以绘制复杂的构象变化或多个分子之间的相互作用。从这样的实验得到的距离可以用于通过三角测量确定荧光团位置。当与荧光团所连接的大分子的已知结构结合时，可以确定系统的三维模型。然而，必须注意从荧光能量转移效率适当地导出距离，并认识到这种关系的系统或随机误差。在这里，我们回顾了用于基于单分子荧光共振转移的三维建模的实验和计算方法，并描述了最近的进展，推动这种方法的限制大分子复合物。
Single molecule fluorescence energy transfer experiments enable investigations of macromolecular conformation and folding by the introduction of fluorescent dyes at specific sites in the macromolecule. Multiple such experiments can be performed with different labeling site combinations in order to map complex conformational changes or interactions between multiple molecules. Distances that are derived from such experiments can be used for determination of the fluorophore positions by triangulation. When combined with a known structure of the macromolecule(s) to which the fluorophores are attached, a three-dimensional model of the system can be determined. However, care has to be taken to properly derive distance from fluorescence energy transfer efficiency and to recognize the systematic or random errors for this relationship. Here we review the experimental and computational methods used for three-dimensional modeling based on single molecule fluorescence resonance transfer, and describe recent progress in pushing the limits of this approach to macromolecular complexes.
登录
查看更多内容
DOI：
10.1007/s00249-008-0383-z
发表时间：
2009-04-01
影响因子：
2
作者：
Cherny, Dmitry I.;Eperon, Ian C.;Bagshaw, Clive R.
通讯作者：
Bagshaw, Clive R.
影响因子：
15
作者：
Brustad EM;Lemke EA;Schultz PG;Deniz AA
通讯作者：
Deniz AA
影响因子：
3.4
作者：
Dave, Richa;Terry, Daniel S.;Blanchard, Scott C.
通讯作者：
Blanchard, Scott C.
影响因子：
3
作者：
DOSREMEDIOS, CG;MOENS, PDJ
通讯作者：
MOENS, PDJ
影响因子：
3.4
作者：
Chung, Hoi Sung;Louis, John M.;Eaton, William A.
通讯作者：
Eaton, William A.