The absolute number of circulating CD34+ cells predicts the number of hematopoietic stem cells that can be collected by apheresis.
The absolute number of circulating CD34+ cells predicts the number of hematopoietic stem cells that can be collected by apheresis.
复制标题
循环CD34细胞的绝对数量预测可以通过单采术收集的造血干细胞的数量。
作者：
R. Schots;I. Riet;Serge Damiaens;J. Flament;P. Lacor;Y. Staelens;L. Steenssens;B. V. Camp;M. Waele
我们报告了我们的单一机构为标准化用于自体移植的外周血祖细胞 (PBPC) 收集方法所做的努力。在 30 名患有各种潜在肿瘤疾病的患者中，通过不同类型的化疗随后使用 G-CSF（24 名患者）或单独使用 G-CSF（6 名患者）来动员 PBPC。使用 CS3000 细胞分离器进行中位数 3 次血浆分离术（范围：2-7），处理的血液体积为 101。我们研究了循环白细胞、单核细胞和 CD34+ 细胞的绝对数量与单次血浆分离术中收集的 PBPC 量之间的关系。通过基于流式细胞术的白细胞亚群分析对 CD34+ 细胞进行定量。单采术产品的 CFU-GM 和 CD34+ 细胞含量（分析了 69 个程序）密切相关：rho = 0.936，P = 0.0001）。回归分析表明，根据血液中CD34+细胞的绝对数可以预测单采血液中CD34+细胞的总数或CFU-GM含量（r=0.915，P=0.0001）。每 mm3 血液中有 10 个循环 CD34+ 细胞，确保当天收集的每公斤至少有 0.5 x 10(6) CD34+ 细胞。在 30 名患者中，17 名患者接受了自体移植，其中 13 名患者仅含有 PBPC。在每公斤回输至少 2 x 10(6) CD34+ 细胞的所有患者中观察到长期且完整的血液学重建。
We report our single institution's effort to standardize the method of collecting peripheral blood progenitor cells (PBPC) used for autologous transplantation. PBPC were mobilized by different types of chemotherapy followed by G-CSF (24 patients) or G-CSF alone (six patients) in 30 patients with various underlying neoplastic diseases. A median of three aphereses (range: 2-7), using the CS3000 cell separator was performed and a blood volume of 101 was processed. We studied the relationship between the absolute numbers of circulating leukocytes, mononuclear cells and CD34+ cells and the amount of PBPC collected during a single apheresis procedure. CD34+ cells were quantified by leukocyte subset analysis based on flow cytometry. Both CFU-GM and CD34+ cell contents of the apheresis products (69 procedures analyzed) correlated strongly: rho = 0.936, P = 0.0001). Regression analysis showed that the total number of CD34+ cells or CFU-GM content of the apheresis products could be predicted (r = 0.915, P = 0.0001) from the absolute number of CD34+ cells in the blood. A number of 10 circulating CD34+ cells per mm3 blood ensured a minimum of 0.5 x 10(6) CD34+ cells per kg, collected on the same day. Of the 30 patients, 17 received an autologous graft that contained only PBPC in 13. Long-term and complete hematological reconstitution was observed in all patients who had a minimum of 2 x 10(6) CD34+ cells per kg reinfused.