J1-160 and J1-180 are oligodendrocyte-secreted nonpermissive substrates for cell adhesion.
J1-160 and J1-180 are oligodendrocyte-secreted nonpermissive substrates for cell adhesion.
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J1-160和J1-180是用于细胞粘附的少突胶质细胞分泌的非允许底物。
DOI：
10.1083/jcb.109.4.1765
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发表时间：
1989-10
影响因子：
7.8
通讯作者：
Schachner, M
中科院分区：
文献类型：
--
作者：
Pesheva, P;Spiess, E;Schachner, M
胶质细胞衍生的J1细胞外基质糖蛋白被称为J1-160/J1-180（发育晚期出现的低分子量组）和J1-200/J1-220（发育早期出现的与腱生蛋白免疫化学相关的高分子组）。两组的成员表现出不同的交叉反应性。为了表征这些J1糖蛋白之间的结构和功能差异，产生了两种单克隆抗体，其仅识别较低分子量组的成员。这两种抗体检测低分子量组成员之间的免疫化学相似性，但不与J1/腱生蛋白反应。J1-160和J1-180由培养物中的分化的少突胶质细胞和中枢神经系统的髓鞘特异性表达，并且在周围神经系统和所测试的成年小鼠的任何其他器官中均未发现。旋转阴影的J1-160和J1-180的电子显微镜检查显示，分别，二聚体和三聚体（tribrachion）扭结臂棒状结构，这是由二硫键连接。J1-160/J1-180是出生后早期小脑小神经元、星形胶质细胞和成纤维细胞附着和扩散的非允许底物。在与层粘连蛋白的混合物中，J1- 160/J1-180是神经元的非允许底物，但不是星形胶质细胞或成纤维细胞的非允许底物。对神经元的排斥作用可以被其中一种单克隆抗体中和，但不能被另一种单克隆抗体中和。这些意见的背景下，在哺乳动物神经系统再生过程中的细胞相互作用进行了讨论。
The glia-derived J1 extracellular matrix glycoproteins have been referred to as J1-160/J1-180 (the developmentally late appearing lower molecular weight group) and J1-200/J1-220 (the developmentally early appearing higher molecular group immunochemically related to tenascin). Members of the two groups show distinct cross-reactivities. To characterize the structural and functional differences between these J1 glycoproteins, two monoclonal antibodies were generated which recognize only the members of the lower molecular weight group. The two antibodies detect immunochemical similarities among the members of the lower molecular weight group, but do not react with J1/tenascin. J1-160 and J1-180 are specifically expressed by differentiated oligodendrocytes in culture and by myelin of the central nervous system and have not been found in the peripheral nervous system nor in any other organ of the adult mice tested. Electron microscopic examination of rotary-shadowed J1-160 and J1-180 reveals, respectively, dimeric and trimeric (tribrachion) kink-armed rodlike structures, which are linked by disulfide bridges. J1-160/J1-180 are nonpermissive substrates for the attachment and spreading of early postnatal small cerebellar neurons, astrocytes, and fibroblasts. In a mixture with laminin, J1- 160/J1-180 are nonpermissive substrates for neurons, but not for astrocytes or fibroblasts. The repulsive effect toward neurons can be neutralized by one of the monoclonal antibodies, but not by the other. These observations are discussed in the context of cell interactions during regeneration in the mammalian nervous system.