Cloning and nucleotide sequence of human gamma-glutamyl transpeptidase.
Cloning and nucleotide sequence of human gamma-glutamyl transpeptidase.
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人γ-谷氨酰转肽酶的克隆和核苷酸序列。
DOI：
10.1073/pnas.85.23.8840
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发表时间：
1988
影响因子：
11.1
通讯作者：
Groffen,J
中科院分区：
文献类型：
--
作者：
Rajpert-DeMeyts,E;Heisterkamp,N;Groffen,J
我们已经确定了人类γ -谷氨酰转肽酶（GGT）的基因；谷氨酰胺：d -谷氨酰肽5-谷氨酰转移酶（也称为γ -谷氨酰转移酶），EC 2.3.2.2]，位于22号染色体q11条带----四分之一的BCR基因相关区域。从噬菌体lambda gt11构建的人胎盘文库中分离出两个与GGT mRNA互补的cdna。最大的cDNA长度为2535个碱基对（bp），开放阅读框包含1707个核苷酸，编码569个氨基酸。利用与该cDNA对应的探针，通过RNA印迹杂交分析，在小鼠肾RNA中检测到约2.4千碱基的mRNA。编码序列编码的GGT前体Mr估计为61400。我们将我们的核苷酸和推断的氨基酸序列与已发表的大鼠肾脏cdna结果进行了比较。人和大鼠的氨基酸序列相似；然而，在180 bp的重链片段中发现了相当大的核苷酸序列差异，导致该区域的氨基酸序列完全不同。此外，人类cDNA的5‘未翻译序列（669 bp）明显大于大鼠cDNA的5’未翻译序列（227 bp）。本研究结果对进一步研究人类GGT的蛋白质结构以及该酶的调控具有一定的参考价值。
We have identified the gene for human gamma-glutamyl transpeptidase [GGT; glutamine:D-glutamyl-peptide 5-glutamyltransferase (also called gamma-glutamyltransferase), EC 2.3.2.2] in a BCR gene-related region located in band q11----qter of chromosome 22. Two cDNAs complementary to the GGT mRNA have been isolated from a human placental library constructed in phage lambda gt11. The largest cDNA has a size of 2535 base pairs (bp) and an open reading frame of 1707 nucleotides encoding 569 amino acids. By using a probe corresponding to this cDNA, a mRNA of approximately 2.4 kilobases was detected by RNA blot-hybridization analysis in mouse kidney RNA. The GGT precursor encoded by the coding sequence would have an estimated Mr of 61,400. We compared our nucleotide and deduced amino acid sequences with the published results of rat kidney cDNAs. The human and rat amino acid sequences are similar; however, a considerable discrepancy in nucleotide sequence was found within a 180-bp fragment of the heavy chain, resulting in a completely different amino acid sequence for this region. In addition, the 5' untranslated sequence of the human cDNA (669 bp) is substantially larger than that determined in the rat cDNA (227 bp). Our results may be valuable for further studies on the protein structure of human GGT as well as studies on the regulation of the enzyme.