Comparing techniques for detecting the number of somatic cells in raw milk
Comparing techniques for detecting the number of somatic cells in raw milk
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DOI：
10.1007/s00217-004-1084-1
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发表时间：
2005-05-01
影响因子：
3.3
通讯作者：
Zheng, XD
中科院分区：
文献类型：
--
作者：
Feng, W;Zheng, XD
评估牛奶质量和监测奶牛乳房炎需要评估体细胞计数（SCC），这是乳制品行业的一个重要参数。本研究采用激光共聚焦显微镜（CLSM）、流式细胞仪（FCM）和光学显微镜（LM）对原料乳的体细胞进行了研究。CLSM法与原料乳中体细胞LM数的相关性良好（r=0.987）。FCM法与LM法相关性良好（r=0.904）。CLSM法与FCM法相关性良好（r=0.908）。本研究表明，CLSM法和FCM法均为体细胞检测提供了快速、简便的方法。
Evaluating the quality of milk and monitoring for cow mastitis requires an evaluation of the somatic cell count (SCC), an important parameter in the dairy industry. We studied the somatic cells of raw milk using a confocal laser scanning microscope (CLSM), flow cytometry (FCM), and a light microscope (LM). The CLSM method gave a good correlation (r=0.987) with the LM counts of somatic cells in raw milk. The FCM method also correlated well (r=0.904) with the LM method. The CLSM method gave a good correlation (r=0.908) with the FCM method. This study shows that the CLSM method and the FCM method both offer rapid and simple approaches to somatic cell detection.