THE METABOLIC DEFECT OF METHIONINE DEPENDENCE OCCURS FREQUENTLY IN HUMAN-TUMOR CELL-LINES
THE METABOLIC DEFECT OF METHIONINE DEPENDENCE OCCURS FREQUENTLY IN HUMAN-TUMOR CELL-LINES
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DOI：
10.1016/0006-291x(83)91218-4
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发表时间：
1983-01-01
影响因子：
3.1
通讯作者：
HOFFMAN, RM
中科院分区：
文献类型：
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作者：
MECHAM, JO;ROWITCH, D;HOFFMAN, RM
蛋氨酸依赖性是指当培养基（Met-Hcy+培养基）中蛋氨酸（Met）被其直接前体同型半胱氨酸（Hcy）取代时，细胞无法生长。迄今为止测试的所有正常未建立的细胞株均不依赖于蛋氨酸，因此在 Met-Hcy+ 培养基中的生长几乎与在 Met-Hcy+ 培养基中一样好。在源自不同类型人类肿瘤的 23 种细胞系中，11 种在 Met-Hcy+ 培养基中根本不生长，并且完全依赖蛋氨酸，而 3 种在该培养基中仅轻微生长。许多测试的肿瘤细胞系除了蛋氨酸依赖性之外几乎没有其他共同点。不同类型的人类肿瘤细胞中蛋氨酸依赖性的高发生率表明蛋氨酸依赖性可能是致癌转化和治疗上可利用的一个重要方面。
Methionine dependence is the inability of cells to grow when methionine (Met) is replaced by its immediate precursor homocysteine (Hcy) in the culture medium (Met-Hcy+ medium). All normal unestablished cell strains tested to date are methionine-independent and thus grow almost as well in Met-Hcy+ medium as they do in Met-Hcy+ medium. Of 23 cell lines derived from diverse types of human tumors, 11 do not grow at all in Met-Hcy+ medium and are absolutely methionine-dependent and 3 grow only slightly in this medium. Many of the tumor cell lines tested have little else in common other than the fact that they are methionine-dependent. The high frequency of occurrence of methionine dependence in diverse types of human tumor cells indicates that methionine dependence may be an important aspect of oncogenic transformation and therapeutically exploitable.