Rapid methods for the study of both stable and unstable plasmids in Pseudomonas.
Rapid methods for the study of both stable and unstable plasmids in Pseudomonas.
复制标题
用于研究假单胞菌中稳定和不稳定质粒的快速方法。
DOI：
10.1099/00221287-124-2-433
复制
发表时间：
1981
期刊：
影响因子：
--
通讯作者：
P. Williams
中科院分区：
文献类型：
--
作者：
R. Wheatcroft;P. Williams
描述了分析假单胞菌中降解质粒的快速方法。用碱性十二烷基硫酸钠制备的细菌裂解物在消泡剂存在下剧烈搅拌。将样品进行琼脂糖凝胶电泳以检测质粒DNA。在蔗糖梯度上短暂离心后，裂解物产生足够纯度的质粒 DNA，用于限制性核酸内切酶消化，无需进一步处理。事实证明，这些方法对于提取以前未检测到的质粒 DNA 最有用，其中一些质粒 DNA 似乎不稳定。
Rapid methods for the analysis of degradative plasmids in Pseudomonas are described. Bacterial lysates prepared with alkaline sodium dodecyl sulphate were vigorously stirred in the presence of antifoam agent. Samples were subjected to agarose gel electrophoresis to detect plasmid DNA. After a short period of centrifugation on a sucrose gradient, the lysates yielded plasmid DNA of sufficient purity for restriction endonuclease digestion without further treatment. The methods have proved most useful in the extraction of previously undetected plasmid DNA, some of which appears to be unstable.