Natural variation and QTL analysis for cationic mineral content in seeds of Arabidopsis thaliana
Natural variation and QTL analysis for cationic mineral content in seeds of Arabidopsis thaliana
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DOI：
10.1111/j.1365-3040.2004.01189.x
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发表时间：
2004-07-01
影响因子：
7.3
通讯作者：
Ernst, WHO
中科院分区：
文献类型：
--
作者：
Vreugdenhil, D;Aarts, MGM;Ernst, WHO
通过筛选一系列种质（生态型）的 Ca、Fe、K、Mg、Mn、Na、Zn 和 P 的总含量，研究了拟南芥种子中阳离子矿物质含量的自然遗传变异。观察到所有矿物质的变异，并且各种矿物质含量之间存在相关性，最明显的是 Ca 和 Mg、P 和 Mg、P 和 Mn 之间的相关性。使用兰茨贝格直立 (Ler) x 佛得角群岛 (Cvi) 重组近交群体，通过 QTL 分析进一步研究了这种变异的遗传基础。对于除 Na 之外的所有矿物质，均检测到一个或多个 QTL，解释了高达 78% 的变异。通过对近等基因系的分析证实了几个QTL的图位位置，这些系在Ler背景中带有小的Cvi基因渗入。由于某些矿物质的积累的生理耦合或不同基因的连锁，QTL 的有趣共定位表明了多效性效应。通过比较QTL的图谱位置和预期在阳离子易位中发挥作用的基因的位置，提出了几个候选基因。
Naturally occurring genetic variation for contents of cationic minerals in seeds of Arabidopsis thaliana was studied by screening a series of accessions (ecotypes) for Ca, Fe, K, Mg, Mn, Na, Zn, and for total contents of P. Variation was observed for all minerals and correlations between contents of various minerals were present, most noticeably between Ca and Mg, P and Mg, and P and Mn. The genetic basis of this variation was further studied by QTL analysis, using the Landsberg erecta (Ler) x Cape Verde Islands (Cvi) recombinant inbred population. For all minerals, except Na, one or more QTL were detected, explaining up to 78% of the variation. The map positions of several QTL were confirmed by analysis of near isogenic lines, carrying small Cvi introgressions in Ler background. Interesting co-locations of QTL suggest pleiotropic effects, due to physiological coupling of the accumulation of certain minerals or to linkage of different genes. By comparing the map positions of QTL with the positions of genes expected to play a role in cation translocation, several candidate genes are suggested.