RAPID SINGLE-DOSE MODEL FOR LUNG-TUMOR INDUCTION IN A/J MICE BY 4-(METHYLNITROSAMINO)-1-(3-PYRIDYL)-1-BUTANONE AND THE EFFECT OF DIET
RAPID SINGLE-DOSE MODEL FOR LUNG-TUMOR INDUCTION IN A/J MICE BY 4-(METHYLNITROSAMINO)-1-(3-PYRIDYL)-1-BUTANONE AND THE EFFECT OF DIET
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DOI：
10.1093/carcin/10.10.1901
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发表时间：
1989-10-01
期刊：
影响因子：
4.7
通讯作者：
CHUNG, FL
中科院分区：
文献类型：
--
作者：
HECHT, SS;MORSE, MA;CHUNG, FL
本文建立了一种相对快速的烟草特异性亚硝胺4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone(NNK)诱导雌性A/J小鼠肺腺瘤的模型。用AIN-76A半合成饲料喂养的小鼠只接受一次腹腔注射。注射2.5、5或10微克分子NNK生理盐水，3-7个月后处死。最大肺肿瘤诱发率(以每只小鼠肺肿瘤数(肿瘤多样性)衡量)发生在3.5个月内。3.5~7个月的肿瘤多发性无明显增加。治疗4个月后，每只小鼠的肺肿瘤数量为11.9。+-。1.0(10微克分子NNK)，3.6+-。0.4(5微克分子)和0.07+-。0.1(生理盐水)。以AIN-76A饮食维持的单剂量10-Mol NNK处理的小鼠肺肿瘤多样性显著较高(8.3±-.0.5)，而用NNK治疗并维持在NIH-07饮食(2.5.+-.0.3)。本研究结果为鉴定可抑制NNK诱导的肺肿瘤形成的化合物提供了一种有用的生物测定方法。
This paper describes the development of a relatively rapid single-dose model for induction of lung adenomas in female A/J mice by the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Mice maintained on AIN-76A semi-synthetic diet were given a single i.p. dose of 2.5, 5 or 10 .mu.mol NNK in saline and killed 3-7 months later. Maximum lung tumor induction, measured by lung tumors per mouse (tumor multiplicity), occurred in 3.5 months. There was no significant incresae in tumor multiplicity between 3.5 and 7 months. Four months after treatment, numbers of lung tumors per mouse were 11.9 .+-. 1.0 (10 .mu.mol NNK), 3.6 .+-. 0.4 (5 .mu.mol) and 0.07 .+-. 0.1 (saline). Lung tumor multiplicity in mice treated with a single dose of 10 .mu.mol NNK and maintained on AIN-76A diet was significantly higher (8.3 .+-. 0.5) than in mice treated with NNK and maintained on NIH-07 diet (2.5 .+-. 0.3). The results of this study establish a useful bioassay for identification of compound that can modify NNK-induced lung tumorigenesis.