Microsclerotia development in Verticillium dahliae: Regulation and differential expression of the hydrophobin gene VDH1
Microsclerotia development in Verticillium dahliae: Regulation and differential expression of the hydrophobin gene VDH1
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DOI：
10.1016/j.fgb.2008.09.014
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发表时间：
2008-12-01
影响因子：
3
通讯作者：
Dobinson, Katherine F.
中科院分区：
文献类型：
--
作者：
Klimes, Anna;Amyotte, Stefan G.;Dobinson, Katherine F.
维管枯萎真菌大丽花黄萎病产生持久的静止结构，称为微核，这对这种植物病原体的长期生存很重要。在此之前，我们发现了一种疏水蛋白基因（VDH1），这是微硬化产生所必需的。目前对VDH1的表达及其调控的研究是为了深入了解与微硬化发育相关的大部分未表征的分子机制。报告基因分析显示，VDH1在小菌核发育、菌丝融合和分生孢子中特异性表达，表明VDH1介导了分生孢子和其他菌丝结构的小菌核发育。我们还报道了营养有效性对大丽花微核发育调控的影响；该基因的活性似乎受到碳供应的调节。最后，VDH1的组成性表达导致疾病症状发展延迟，但对体外微硬化发育无明显影响。英国皇家版权所有(C) 2008 Elsevier Inc.出版版权所有。
The vascular wilt fungus Verticillium dahliae produces persistent resting structures, known as microsclerotia, which are important for this plant pathogen's long-term survival. Previously, we identified a hydrophobin gene (VDH1) that is necessary for microsclerotial production. The current study of VDH1's expression, and its regulation, was undertaken to provide insight into the largely uncharacterized molecular mechanisms relevant to microsclerotial development. Reporter gene analysis showed that VDH1 is specifically expressed in developing microsclerotia, as well as in hyphal fusions and conidiophores, suggesting that VDH1 mediates the development of microsclerotia from conidiophores and other hyphal structures. We report also on the effects of nutrient availability on the regulation of microsclerotial development in V. dahliae; the gene's activity appears to be regulated in response to carbon availability. Lastly, constitutive expression of VDH1 results in delayed disease symptom development, but has no noticeable effect on in vitro microsclerotial development. Crown Copyright (C) 2008 Published by Elsevier Inc. All rights reserved.