Distinction of intrahepatic metastasis from multicentric carcinogenesis in multifocal hepatocellular carcinoma using molecular alterations.
Distinction of intrahepatic metastasis from multicentric carcinogenesis in multifocal hepatocellular carcinoma using molecular alterations.
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DOI：
10.1016/j.humpath.2017.11.011
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发表时间：
2018-03
期刊：
影响因子：
3.3
通讯作者：
Wood LD
中科院分区：
文献类型：
--
作者：
Chianchiano P;Pezhouh MK;Kim A;Luchini C;Cameron A;Weiss MJ;He J;Voltaggio L;Oshima K;Anders RA;Wood LD
肝细胞癌（HCC）患者经常有多个解剖学上不同的肿瘤。在这些患者中，多灶性HCC可能代表单一癌症的肝内转移（IM）或多中心癌变（MC）伴多个独立肿瘤。为了确定这两种可能性的频率和临床意义，我们对来自24名患者的70例解剖学上不同的HCC进行了组织学和分子分析。我们通过桑格测序分析了TERT启动子区的突变，并使用下一代测序分析了7个充分表征的HCC驱动基因的整个编码区-基于这些基因中的共享或不一致突变，我们将每个患者的HCC分类为IM、MC或不确定。在我们的队列中，TERT启动子的突变是最常见的改变，存在于71%的分析肿瘤中。其余基因的突变发生在不到20%的分析肿瘤中。我们能够确定在58%的患者分析的相关性：MC发生在41%的患者中，33%与单纯MC和8%与MC和IM。IM仅发生在17%的患者中，而其余患者则不确定。这项研究强调了分子分析在确定多灶性HCC相关性方面的实用性;然而，靶向测序只能解决大约60%的多灶性HCC患者的这种差异。
Patients with hepatocellular carcinoma (HCC) frequently have multiple anatomically distinct tumors. In these patients, multifocal HCC could represent intrahepatic metastases (IMs) of a single cancer or multicentric carcinogenesis (MC) with multiple independent neoplasms. To determine the frequency and clinical implications of these 2 possibilities, we performed histological and molecular analysis of 70 anatomically distinct HCCs from 24 patients. We assayed mutations in the TERT promoter region by Sanger sequencing and used next-generation sequencing to analyze the entire coding regions of 7 well-characterized HCC driver genes—based on shared or discordant mutations in these genes, we classified the HCCs in each patient as IM, MC, or indeterminate. Mutations in the TERT promoter were the most common alteration in our cohort, present in 71% of tumors analyzed. Mutations in the remaining genes occurred in less than 20% of analyzed tumors. We were able to determine the relatedness in 58% of the patients analyzed: MC occurred in 41% of patients, with 33% with exclusively MC and 8% with both MC and IM. IM occurred exclusively in 17% of patients, whereas the remainder were indeterminate. This study highlights the utility of molecular analyses to determine relatedness in multifocal HCC; however, targeted sequencing can only resolve this distinction in approximately 60% of patients with multifocal HCC.
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影响因子：
81.5
作者：
Llovet, Josep M.;Zucman-Rossi, Jessica;Gores, Gregory
通讯作者：
Gores, Gregory
使用多组学平台识别肝胆和胰腺系统中同步多灶性肿瘤的克隆起源。
影响因子：
--
作者：
Jiang W;Ding Y;Shen Y;Fan L;Zhou L;Li Z;Zheng Y;Zhao P;Liu L;Tong Z;Fang W;Wang W
通讯作者：
Wang W
影响因子：
64.5
作者：
Cancer Genome Atlas Research Network. Electronic address: wheeler@bcm.edu;Cancer Genome Atlas Research Network
通讯作者：
Cancer Genome Atlas Research Network
影响因子：
7.3
作者：
Wu, Ren-Chin;Ayhan, Ayse;Maeda, Daichi;Kim, Kyu-Rae;Clarke, Blaise A.;Shaw, Patricia;Chui, Michael Herman;Rosen, Barry;Shih, Ie-Ming;Wang, Tian-Li
通讯作者：
Wang, Tian-Li
DOI：
10.1016/j.jamcollsurg.2013.07.402
发表时间：
2013-12-01
影响因子：
5.2
作者：
Wang, Bin;Xia, Chun-Yan;Wu, Meng-Chao
通讯作者：
Wu, Meng-Chao