An siRNA-based system for differential regulation of ectopic gene expression constructs
An siRNA-based system for differential regulation of ectopic gene expression constructs
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DOI：
10.1016/j.jbiotec.2006.12.015
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发表时间：
2007-03-10
影响因子：
4.1
通讯作者：
Bielke, Wolfgang
中科院分区：
文献类型：
--
作者：
Hahn, Peter;Niemann, Gesa;Bielke, Wolfgang
我们描述了一种基于siRNA(短干扰RNA)的方法，用于暂时和可逆地调节细胞中的工程表达结构。对克隆基因的控制可以通过与作为表达载体组成部分的人工靶序列互补的独特的siRNAs的共转染来实现。这种方法的应用允许在同一细胞内同时或相互差异地调节两个或更多的基因结构，减少不必要的副作用。与使用化合物的启动子调控系统相比，这种方法有几个优点。(C)2007 Elsevier B.V.保留所有权利。
We describe an siRNA (short interfering RNA)-based approach for temporary and reversible regulation of engineered expression constructs in cells. Control of cloned genes can be achieved by cotransfection of unique siRNAs, complementary to artificial target sequences, which are integral parts of an expression vector. Application of this method allows simultaneous or mutual-differential regulation of two or more gene constructs within the same cell, reducing unwanted side effects. This method provides several advantages over promoter regulatory systems employing chemical compounds. (c) 2007 Elsevier B.V. All rights reserved.