Regulation of protein synthesis factor EF-1 alpha in Mucor racemosus.
Regulation of protein synthesis factor EF-1 alpha in Mucor racemosus.
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总状毛霉中蛋白质合成因子 EF-1 α 的调节。
DOI：
10.1128/mcb.5.5.1100-1103.1985
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发表时间：
1985
影响因子：
5.3
通讯作者：
Sypherd,PS
中科院分区：
文献类型：
--
作者：
Fonzi,WA;Katayama,C;Leathers,T;Sypherd,PS
总状毛霉蛋白合成延伸因子EF-1α每分子含有8 ~ 9个甲基化氨基酸，而孢子囊孢子蛋白合成延伸因子EF-1 α则没有甲基化。在孢子萌发过程中，粗提物中该因子的比活性增加了6倍。这种活性增加伴随着恒定水平的EF-1α特异性mRNA和恒定水平的EF-1α蛋白。然而，甲基化的蛋白质，加速在萌发过程中，在平行的因子的比活性的增加。我们认为，EF-1α的活性在萌发过程中通过蛋白质的甲基化进行调节，而不涉及转录调控。
The protein synthesis elongation factor EF-1α ofMucor racemosushyphae contained eight or nine methylated amino acids per molecule, whereas the factor from sporangiospores was nonmethylated. During the course of spore germination, the specific activity of the factor in crude extracts increased sixfold. This increase in activity was accompanied by a constant level of EF-1α-specific mRNA and a constant level of EF-1α protein. Methylation of the protein, however, accelerated during the germination process, in parallel with the increase in specific activity of the factor. We propose that the activity of EF-1α is regulated during germination through methylation of the protein and does not involve transcriptional regulation.