Identifying Vulnerable Pathways in Mycobacterium tuberculosis by Using a Knockdown Approach
Identifying Vulnerable Pathways in Mycobacterium tuberculosis by Using a Knockdown Approach
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DOI：
10.1128/aem.02880-10
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发表时间：
2011-07-01
影响因子：
4.4
通讯作者：
Parish, Tanya
中科院分区：
文献类型：
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作者：
Carroll, Paul;Faray-Kele, Marie-Claire;Parish, Tanya
我们构建了特定基因表达下调的结核分枝杆菌重组菌株，以识别易受攻击的药物靶点。根据巨噬细胞和培养物的生长表型对靶点进行排序：dprE1、clpP1和fadD32操纵子是最好的靶点，glnA1、glnE、pKnL、regX3和senX3是较差的靶点。
We constructed recombinant strains of Mycobacterium tuberculosis in which expression of specific genes was downregulated to identify vulnerable drug targets. Growth phenotypes in macrophages and culture were used to rank targets: the dprE1, clpP1, and fadD32 operons were the best targets and glnA1, glnE, pknL, regX3, and senX3 were poor targets.