Formation and stability of a Janus-Wedge type of DNA triplex
Formation and stability of a Janus-Wedge type of DNA triplex
复制标题
DOI：
10.1021/ja038081x
复制
发表时间：
2004-01-14
影响因子：
15
通讯作者：
McLaughlin, LW
中科院分区：
文献类型：
--
作者：
Chen, DL;Meena;McLaughlin, LW
描述了一种新型的具有Janus-Wedge（J-W）三螺旋形成的DNA靶向。连接到PNA主链上的“楔形”残基（W）被设计成将其自身插入双链DNA中，并与两个沃森-克里克面进行碱基配对。为了研究这种组装的稳定性，我们研究了具有dC 8 − T8靶序列的J-W三链体的形成。使用这种靶序列可以研究这种新的螺旋形式，而不会在两个靶残基之间产生竞争性的沃森-克里克相互作用。研究表明，W链与两条靶链结合，具有确定的极性和稳定性（−15.2 kcal/mol），大致为两个独立的双链相互作用的总和。
A new type of DNA targeting with the formation of a Janus-Wedge (J-W) triple helix is described. The “wedge” residue (W) attached to a PNA backbone is designed to insert itself into double-stranded DNA and base pair with both Watson−Crick faces. To study the stability of such an assembly, we have examined the formation of the J-W triplex with dC8− T8target sequence. The use of this target sequence permits the study of this new helix form without competing Watson−Crick interactions between the two target residues. Studies indicate that the W strand binds to both target strands, with defined polarity and a stability (−15.2 kcal/mol) that is roughly the sum of the two independent duplex interactions.