Characterisation and biofilm screening of the predominant bacteria isolated from whey protein concentrate 80

Characterisation and biofilm screening of the predominant bacteria isolated from whey protein concentrate 80
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DOI:
10.1007/s13594-015-0264-z
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发表时间:
2016-03
影响因子:
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通讯作者:
Siti Norbaizura Md Zain;S. Flint;R. Bennett;Hong-Soon Tay
Siti Norbaizura Md Zain;S. Flint;R. Bennett;Hong-Soon Tay
中科院分区:
工程技术2区
文献类型:
--
作者:
Siti Norbaizura Md Zain;S. Flint;R. Bennett;Hong-Soon Tay

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The source of microbiological contamination of whey protein concentrate (WPC), a quality problem for the dairy industry, has not been thoroughly investigated. The objectives of this study were to identify the bacteria isolated from whey protein concentrate containing 80% protein (WPC 80) and determine their ability to form biofilms as a potential source of contamination in a whey processing line. SixBacillusspecies including (percentage of isolates in brackets)Bacillus licheniformis(67%),Bacillus cereus(19%),Bacillus thuringensis(4%),Bacillus subtilis(4%),Bacillus pumilus(4%) andPaenibacillus glucanolyticus(2%) were identified based on BLAST databases in six different WPC80 batches.B. licheniformiswas the predominant isolate.B. licheniformisare thermo-tolerant bacteria with the ability to form biofilm. This biofilm is a potential source of microbial contamination of product, resulting in microbial specification limits being exceeded. Spoilage of product may result from the metabolic products, such as enzymes, produced by these biofilms.