Component Profiling in Agricultural Applications Using an Adjustable Acupuncture Needle for Sheath-Flow Probe Electrospray Ionization/Mass Spectrometry
Component Profiling in Agricultural Applications Using an Adjustable Acupuncture Needle for Sheath-Flow Probe Electrospray Ionization/Mass Spectrometry
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可调针鞘流探针电喷雾质谱在农业应用中的组分分析
DOI：
10.1021/acs.jafc.8b06424
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发表时间：
2019-03-20
影响因子：
6.1
通讯作者：
Nonami, Hiroshi
中科院分区：
文献类型：
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作者：
Hiraoka, Kenzo;Rankin-Turner, Stephanie;Nonami, Hiroshi
在以往的工作中，探针式电喷雾电离/质谱仪(PESI/MS)和鞘流探针式电喷雾电离/质谱仪(sfPESI/MS)被报道用于食品的快速和微创分析。在这项工作中，将报告sfPESI的修改版本。将针刺针插入从尖端伸出5 mm的sfPESI探头中，刺穿样品表面。针刺入标本的深度约为1 mm。采样后，将针收回到溶剂预装载毛细管中，突出长度为距针尖0.1~0.2 mm。通过在针上施加高电压，获得了针上捕获的样品的质谱图。该方法适用于SF-PESI难以分析的表面覆盖有表皮层蜡层的植物的仿形分析。对大田生长的杏树进行了现场质谱分析，以监测果实的发育阶段。
In previous work, probe electrospray ionization/mass spectrometry (PESI/MS) and sheath-flow probe electrospray ionization/mass spectrometry (sfPESI/MS) were reported for the rapid and minimally invasive analysis of food. In this work, a modified version of sfPESI will be reported. The sample surface was pricked with an acupuncture needle inserted in the sfPESI probe that protruded from the terminus of the tip by 5 mm. The invasion depth of the needle into the sample was similar to 1 mm. After sampling, the needle was retracted into the solvent-preloaded capillary with a protrusion length of 0.1-0.2 mm from the tip. A mass spectrum of the sample captured on the needle was obtained by applying a high voltage to the needle. This method could be applicable to profiling analyses of plants with the epicuticular wax covering on the surfaces that are difficult to analyze by sf-PESI. The on-site mass spectrometric analysis for a growing apricot in the field was performed to monitor the developing stage of the fruit.