Effects of Tranexamic Acid Cytotoxicity on In Vitro Chondrocytes.
Effects of Tranexamic Acid Cytotoxicity on In Vitro Chondrocytes.
复制标题
氨甲环酸对体外软骨细胞的细胞毒性作用。
DOI：
--
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
M. Ehrlich
中科院分区：
文献类型：
--
作者：
John R Tuttle;Peter Feltman;Scott A. Ritterman;M. Ehrlich
在过去的十年中，局部氨甲环酸（TXA）在骨科手术中的使用不断扩大，越来越多的证据证实围手术期失血量和输血需求减少，但关于TXA对天然软骨的影响的证据很少。我们进行了一项研究，以了解TXA对牛软骨和鼠软骨细胞的体外作用，并最终扩大局部TXA的临床应用，包括保留天然软骨的情况，如半关节成形术。将牛软骨外植体暴露于浓度为100 mg/mL的TXA，并在8、24和48小时测量糖胺聚糖（GAG）释放和细胞活力。将单层鼠软骨细胞暴露于TXA 25、50和100 mg/mL，并在8、24和48小时测量活力。在所有时间点，暴露于TXA 100 mg/mL的样品中，牛外植体释放的GAG显著更高。在初始孵育后24和48小时暴露于TXA的外植体中细胞活力显著降低。牛软骨细胞活力不受TXA 25 mg/mL的影响。在所有时间点，TXA 25 mg/mL和对照样品之间的小鼠软骨细胞活力相似。TXA 50 mg/mL样品从8小时的66.51%活力降至24小时的6.81%活力，并在48小时时完全细胞死亡。TXA 100 mg/mL样品在8、24和48小时时未观察到活细胞。我们的数据表明，TXA 100 mg/mL损伤和细胞毒性的牛软骨和小鼠软骨细胞的细胞毒性。TXA 25 mg/mL不影响小鼠和牛软骨细胞活力。
Use of topical tranexamic acid (TXA) in orthopedic surgery has been expanding over the past decade, with increasing evidence confirming reductions in perioperative blood loss and transfusion requirements, but there is minimal evidence regarding effects of TXA on native cartilage. We conducted a study to understand the in vitro effects of TXA on bovine cartilage and murine chondrocytes and ultimately to expand the clinical application of topical TXA to include scenarios with retained native cartilage, such as hemiarthroplasty. Bovine cartilage explants were exposed to TXA at a concentration of 100 mg/mL, and glycosaminoglycan (GAG) release and cell viability were measured at 8, 24, and 48 hours. Monolayer murine chondrocytes were exposed to TXA 25, 50, and 100 mg/mL, and viability was measured at 8, 24, and 48 hours. GAG released from bovine explants was significantly higher in the samples exposed to TXA 100 mg/mL at all time points. Cell viability was significantly decreased in the explants exposed to TXA 24 and 48 hours after initial incubation. Bovine chondrocyte viability was not affected by TXA 25 mg/mL. Murine chondrocyte viability was similar between the TXA 25 mg/mL and control samples at all time points. The TXA 50 mg/mL sample dropped from 66.51% viability at 8 hours to 6.81% viability at 24 hours and complete cell death by 48 hours. The TXA 100 mg/mL samples had no observable viable cells at 8, 24, and 48 hours. Our data indicated that TXA 100 mg/mL damaged and was cytotoxic to bovine explanted cartilage and was cytotoxic to murine chondrocytes. Murine and bovine chondrocyte viability were not affected by TXA 25 mg/mL.
影响因子：
6.9
作者：
LEFEBVRE, V;GAROFALO, S;DECROMBRUGGHE, B
通讯作者：
DECROMBRUGGHE, B
影响因子：
3.5
作者：
Konig, Gerhardt;Hamlin, Brian R.;Waters, Jonathan H.
通讯作者：
Waters, Jonathan H.