The 1-Carbamoyl-2-oxo-4,5-dihydroxyimidazolidine Component of ROS-Induced DNA Damage in White Blood Cells
The 1-Carbamoyl-2-oxo-4,5-dihydroxyimidazolidine Component of ROS-Induced DNA Damage in White Blood Cells
复制标题
DOI：
10.1667/rr2087.1
复制
发表时间：
2010-07-01
影响因子：
3.4
通讯作者：
Box, Harold C.
中科院分区：
文献类型：
--
作者：
Iijima, Herbert;Patrzyc, Helen B.;Box, Harold C.
胞嘧啶的1-氨基甲酰基-2-氧代-4，5-二羟基咪唑烷修饰是已知的通过氧化应激在体外产生的碱基修饰。然而，这种修饰在体内的存在尚未确定。在这项研究中，这种碱修饰引入到二核苷单磷酸是使用芬顿反应完成的。随后，在短的同位素标记的低聚物中产生修饰。将带有损伤的标记四聚体用作LC-MS/MS测定来自健康供体的白色血细胞DNA中碱基修饰的内标。发现胞嘧啶的1-氨基甲酰基-2-氧代4，5-二羟基咪唑烷修饰的背景水平大于甲酰胺和胸腺嘧啶乙二醇碱基修饰的水平。(C)2010年，辐射研究学会
The 1-carbamoyl-2-oxo-4,5-dihydroxyimidazolidine modification of cytosine is a known base modification produced in vitro by oxidative stress. However, the presence of this modification in vivo has not been established. In this study the introduction of this base modification into dinucleoside mono-phosphates was accomplished using the Fenton reaction. Subsequently, the modification was produced in short isotopically labeled oligomers. Labeled tetramers bearing the lesion were used as internal standards for LC-MS/MS determinations of the base modification in the DNA of white blood cells from healthy donors. The background level of the 1-carbamoyl-2-oxo4,5-dihydroxyimidazolidine modification of cytosine was found to be larger than the levels of the formamide and thymine glycol base modifications. (C) 2010 by Radiation Research Society