Complete genomic sequence analysis of a highly virulent isolate revealed a novel strain of Sugarcane mosaic virus
Complete genomic sequence analysis of a highly virulent isolate revealed a novel strain of Sugarcane mosaic virus
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对高毒力分离株的完整基因组序列分析揭示了甘蔗花叶病毒的新毒株
DOI：
10.1007/s11262-011-0644-2
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发表时间：
2011-12-01
期刊：
影响因子：
1.6
通讯作者：
Miao, Hong-Qin
中科院分区：
文献类型：
--
作者：
Gao, Bo;Cui, Xiao-Wen;Miao, Hong-Qin
甘蔗花叶病毒（SCMV）是引起我国北方玉米矮花叶病的最常见病毒。从保定地区表现矮化和花叶症状的玉米上分离到一个SCMV分离物BD 8。BD 8的完整基因组序列为9，576个核苷酸（nt），不包括聚（A）尾。该基因包含一个长9，192 nt的开放阅读框，编码一个长3，063个氨基酸的多聚蛋白，其5 '端非翻译区（UTR）长148 nt，3'端非翻译区（UTR）长236 nt。BD 8与GenBank中已发表的13个SCMV分离物的全基因组核苷酸序列同源性为79.1%~ 80.8%，CP基因核苷酸和氨基酸序列同源性分别为76.9%~ 82.6%和82.8%~ 86.9%。系统发育分析表明，SCMV可分为4个类群：类群I包括玉米分离物，类群II包括甘蔗或玉米分离物，类群III和IV分别包括AU-A（AJ 278405）和BD 8。因此，BD 8代表SCMV的新菌株。进一步对更多分离株的CP基因序列分析表明，BD 8与泰国、越南分离株聚为一类，表明该菌株在南亚地区有分布。在温室条件下，BD 8对12个供试玉米品种均能引起严重症状，且发病率较高，表明BD 8具有强致病力。
Sugarcane mosaic virus (SCMV) is the most prevalent virus causing maize dwarf mosaic disease in northern China. A SCMV isolate, BD8, was obtained from the maize showing dwarf and mosaic symptoms in Baoding, China. The complete genomic sequence of BD8 is 9,576 nucleotides (nt) excluding the poly(A) tail. It contains one single open reading frame of 9,192 nt and encodes a large polyprotein of 3,063 amino acids (aa), flanked by a 5'-untranslated region (UTR) of 148 nt and a 3'-UTR of 236 nt. The entire genomic sequence of BD8 shares identities of 79.1-80.8% with those of other 13 SCMV isolates available in the GenBank at nt level, while their CP genes share identities of 76.9-82.6 and 82.8-86.9% at nt and aa levels, respectively. Phylogenetic analysis of the complete genomic sequences reveals that SCMV can be clustered to four groups: group I includes isolates from maize, group II consists of isolates from sugarcane or maize, groups III and IV contain single isolate of AU-A (AJ278405) and BD8, respectively. Thus, BD8 represents a new strain of SCMV. Furthermore analysis of the CP gene sequences of more isolates shows that BD8 is clustered to a group with the isolates from Thailand and Vietnam, which implies that isolates of this strain have been distributed in South Asia. In the greenhouse, BD8 can cause severe symptoms in all the 12 maize varieties tested with high incidence, indicating that BD8 is highly virulent.