Elastic incoherent neutron scattering as a probe of high pressure induced changes in protein flexibility
Elastic incoherent neutron scattering as a probe of high pressure induced changes in protein flexibility
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DOI：
10.1016/j.bbapap.2009.08.025
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发表时间：
2010-01-01
影响因子：
3.2
通讯作者：
Di Venere, A.
中科院分区：
文献类型：
--
作者：
Filabozzi, A.;Deriu, A.;Di Venere, A.
本文报道了三种球状蛋白质(胰酶、溶菌酶和β-乳球蛋白)在1bar到5.5kbar的不同压力区间内的弹性非相干中子散射实验结果。随着压力的增加，均方氢的起伏减小。胰酶和β-乳球蛋白的行为相似，而溶菌酶在~lt；u(2)和gt；中的变化要大得多。这可能与变性过程中的不同步骤和溶菌酶形成淀粉样蛋白的高度倾向有关。弹性非相干中子散射已被证明是研究压力引起的蛋白质柔韧性变化的一种有效的微观技术。(C)2009爱思唯尔B.V.保留所有权利。
We report here the results of elastic incoherent neutron scattering experiments on three globular proteins (trypsin, lysozyme and beta-lactoglobulin) in different pressure intervals ranging from 1 bar to 5.5 kbar. A decrease of the mean square hydrogen fluctuations, < u(2)>, has been observed upon increasing pressure. Trypsin and beta-lactoglobulin behave similarly while lysozyme shows much larger changes in < u(2)>. This can be related to different steps in the denaturing processes and to the high propensity of lysozyme to form amyloids. Elastic incoherent neutron scattering has proven to be an effective microscopic technique for the investigation of pressure induced changes in protein flexibility. (C) 2009 Elsevier B.V. All rights reserved.