Production and specificity of a monoclonal anti-11-deoxycortisol antibody.
Production and specificity of a monoclonal anti-11-deoxycortisol antibody.
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单克隆抗 11-脱氧皮质醇抗体的产生和特异性。
DOI：
10.1248/cpb.34.2914
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发表时间：
1986
影响因子：
1.7
通讯作者：
T. Nambara
中科院分区：
文献类型：
--
作者：
H. Hosoda;N. Kobayashi;S. Tamura;M. Mitsuma;J. Sawada;T. Terao;T. Nambara
描述了11-脱氧皮质醇的单克隆抗体的生产。用4-（2-羧乙硫基）-1/1-脱氧皮质醇（4-（2-carboxyethylthio）-1/1-deoxycortisol）与牛血清白蛋白（BSA）偶联免疫BALB/c小鼠，取其脾细胞与P3-NS 1/1-Ag 4 -1骨髓瘤细胞融合。建立了一株分泌对11-脱氧皮质醇具有高亲和力（Ka=2× 1010 M-1）的免疫球蛋白G1（IgG 1）的杂交瘤克隆（S.CET.M8.1.1）。用放射免疫分析法测定人血浆标本中11-脱氧皮质醇的含量，并与常规的多克隆抗血清作比较，以评价单克隆抗体（CET-M8）的特异性。结果表明，该单克隆抗体具有较好的特异性，可用于甲吡酮试验中血浆11-脱氧皮质醇水平的测定，并对影响单克隆抗体特异性的因素进行了讨论。
The production of a monoclonal antibody to 11-deoxycortisol is described. Spleen cells from BALB/c mice immunized with 4-(2-carboxyethylthio)-11-deoxycortisol linked to bovine serum albumin were fused with P3-NS1/1-Ag4-1 myeloma cells. A hybridoma clone (S.CET.M8.1.1) was established that secreted immunoglobulin G1 (IgG1) with a high affinity for 11-deoxycortisol (Ka=2×1010M-1). The specifictity of the monoclonal antibody (CET-M8) was assessed by crossreaction studies wiht closely related steroids and by measuring the amount of 11-deoxycortisol in human plasma specimens by means of radioimmunoassay, in comparison with that of a conventional polyclonal antiserum. The results showed that the monoclonal antibody is reasonably specific and useful for the determination of plasma 11-deoxycortisol levels in the metyrapone test.Factors influencing the specificity of monoclonal antibodies to steroids are discussed.