Incidence and Diversity of Double-Stranded RNAs Occurring in the Chestnut Blight Fungus, Cryphonectria parasitica, in China and Japan.
Incidence and Diversity of Double-Stranded RNAs Occurring in the Chestnut Blight Fungus, Cryphonectria parasitica, in China and Japan.
复制标题
中国和日本板栗疫病真菌 Cryphonectria parasitica 中双链 RNA 的发生率和多样性。
DOI：
10.1094/phyto.1998.88.8.811
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发表时间：
1998
期刊：
影响因子：
3.2
通讯作者：
M. Milgroom
中科院分区：
文献类型：
--
作者：
T. Peever;Y. C. Liu;K. Wang;B. Hillman;R. Foglia;M. Milgroom
摘要从中国栗疫病菌10个亚群和日本栗疫病菌8个亚群中随机抽取栗疫病菌分离株，用双链RNA特异性单克隆抗体进行免疫印迹检测。dsRNA在C.中国和日本的寄生虫感染率分别为2%和6%，远低于此前在北美人群中发现的28%。利用RNA-RNA杂交技术和双链RNA探针研究了中国和日本群体内和群体间的遗传相关性。大多数中国和日本的dsRNA是一个单一杂交组的成员，与来自欧洲的Cryphonectria hypovirus 1（CHV1）有关，被称为CHV1型dsRNA。没有证据表明在中国和日本采样的CHV1型dsRNA之间存在遗传分化。五个日本分离株含有两个遗传上不同的dsRNA。较大的片段（约12个双链酶[kb]）是CHV 1杂交组的成员，而较小的片段（约3 kb）不与来自C.寄生物的2.7-kb dsRNA，包括来自新泽西的分离物NB 631的2.7-kb dsRNA或来自密歇根的分离物RC 1的dsRNA。来自中国东北辽宁省的一个分离物的两个小的dsRNA片段（约1.8和2kb）不与任何测试的dsRNA探针杂交，包括来自C.寄生在北美洲。来自中国安徽省的三个dsRNA与栗疫病菌低病毒2（CHV2）特异性探针杂交，因此被称为CHV2型dsRNA。对安徽分离的3株CHV2型dsRNA进行1,627bp的序列分析，结果表明，这3株CHV2型dsRNA在所测区域内完全相同，与分离自新泽西的CHV2-NB 58有很近的亲缘关系。我们推测CHV2-NB58可能是从中国的这一地区传入北美的。这是首次记录到的北美C.寄生虫dsRNA与来自亚洲的dsRNA遗传相关。
ABSTRACT Isolates of the chestnut blight fungus, Cryphonectria parasitica, were randomly sampled from 10 subpopulations in China and 8 subpopulations in Japan and screened for the presence of double-stranded (ds) RNA using an immunoblot procedure with a monoclonal antibody specific for dsRNA. The overall incidence of dsRNA in C. parasitica was 2 and 6% in China and Japan, respectively, much lower than the 28% found previously in North American populations. Genetic relatedness of dsRNAs within and among populations in China and Japan was examined using RNA-RNA hybridizations with labeled-dsRNA probes. The majority of Chinese and Japanese dsRNAs were members of a single hybridization group, related to Cryphonectria hypovirus 1 (CHV1) from Europe, and are referred to as CHV1-type dsRNAs. No evidence was obtained for genetic differentiation between CHV1-type dsRNAs sampled in China and Japan. Five Japanese isolates contained two genetically distinct dsRNAs. The larger segments (approximately 12 kilobases [kb]) were members of the CHV1 hybridization group, while the smaller segments (approximately 3 kb) did not hybridize with any known dsRNA from C. parasitica including the 2.7-kb dsRNA from isolate NB631 from New Jersey or dsRNA from isolate RC1 from Michigan. Two small dsRNA segments (approximately 1.8 and 2 kb) from one isolate sampled from Liaoning Province in northeastern China did not hybridize with any of the dsRNA probes tested including several described dsRNAs of similar size from C. parasitica in North America. Three dsRNAs from Anhui Province, China, hybridized to Cryphonectria hypovirus 2 (CHV2)-specific probes and are thus referred to as CHV2-type dsRNAs. Sequence analysis of 1,627 base pairs of these three CHV2-type dsRNAs from Anhui revealed that they were identical to each other in the region sequenced and very closely related to CHV2-NB58, isolated from New Jersey. We speculate that CHV2-NB58 may have been introduced into North America from this part of China. This is the first record of a North American C. parasitica dsRNA that is genetically related to a dsRNA from Asia.