Method for monitoring urea and ammonia in wine and must by flow injection-pervaporation
Method for monitoring urea and ammonia in wine and must by flow injection-pervaporation
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DOI：
10.1016/s0003-2670(02)00872-3
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发表时间：
2002-10-23
影响因子：
6.2
通讯作者：
de Castro, MDL
中科院分区：
文献类型：
--
作者：
González-Rodríguez, J;Pérez-Juan, P;de Castro, MDL
建立了一种易于自动化的流动注射-渗透蒸发法，用于监测葡萄酒和葡萄酒中的尿素和氨。该方法是通过渗透蒸发将氨从样品基质中分离出来，然后与水杨酸盐、次氯酸盐和硝普苷反应形成重氮盐，在647 nm处最大吸收。在渗透汽化之前，尿素必须由脲酶催化转化为氨。根据需要通过单变量或多变量方法进行优化后，建立了两种分析物的线性范围（在0和25 mg l（-1）之间）。然后，从重复性（分别为0.52和0.43 mg l(-1)）、重现性（分别为1.34和1.21 mg l(-1)）、检出限和定量限（LOD分别为0.9和0.6 mg l（-1）、LQ分别为1.02和0.67 mg l(-1)）和可追溯性等方面对该方法与参考方法进行了评价。样品吞吐量为16个样品h（-1）。该方法可用于对陈酒和幼酒中的目标分析物进行监测，以控制其含量，防止氨基甲酸乙酯的形成。(C) 2002 Elsevier Science B.V.版权所有
An easy to automate flow injection-pervaporation method for monitoring urea and ammonia in must and wine was developed. The method is based on separation of the ammonia from the sample matrix by pervaporation followed by its reaction with salicylate, hypochlorite and nitroprusside to form a diazonium salt with maximum absorption at 647 nm. Conversion of urea into ammonia catalysed by urease was mandatory before pervaporation. After optimisation by either the univariate or multivariate approaches as required, the linear range was established (between 0 and 25 mg l(-1)) for both analytes. Then, the assessment of the proposed method versus a reference one for urea and ammonia was studied in terms of repeatability (0.52 and 0.43 mg l(-1), respectively), reproducibility (1.34 and 1.21 mg l(-1), respectively), detection and quantification limits (LOD = 0.9 and 0.6 mg l(-1), LQ = 1.02 and 0.67 mg l(-1), respectively) and traceability. The sample throughput was 16 samples h(-1). The method can be applied to the monitoring of the target analytes in must and young wine in order to control their contents, preventing formation of ethyl carbamate. (C) 2002 Elsevier Science B.V. All rights reserved.