Non-invasive intraoperative monitoring of blood pressure and arterial pCO2 during surgical anesthesia in mice
Non-invasive intraoperative monitoring of blood pressure and arterial pCO2 during surgical anesthesia in mice
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DOI：
10.1016/j.jneumeth.2006.07.016
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发表时间：
2007-01-30
影响因子：
3
通讯作者：
Plesnila, Nikolaus
中科院分区：
文献类型：
--
作者：
Thal, Serge C.;Plesnila, Nikolaus
尽管基因工程小鼠在生物医学研究中的使用不断增加，但由于缺乏可逆动脉插管技术，在暂时麻醉的小鼠中难以实现对关键生理参数（如血压和动脉血气）的控制。在这里，我们报告了使用非侵入性技术可以可靠地测量麻醉和人工通气小鼠的动脉血压和血气。C57 B16小鼠通过腹腔注射咪达唑仑、芬太尼和美托咪定麻醉，插管和通气3 h。通过微量二氧化碳测定法监测潮气末pCO（2）。无创血压（NIBP）与颈外动脉有创血压（r = 0.99，P < 0.001）、潮气末PCO2与动脉血PCO2（r= 0.93，P < 0.001）呈高度相关。目前的结果表明，它是可能的，以可靠地测量和控制最相关的生理参数在麻醉小鼠。因此，目前的研究可能有助于减少动物数量，并在未来更明确和控制的生理条件下进行小鼠实验。(c)2006 Elsevier B.V.保留所有权利。
Despite the constantly increasing use of genetically engineered mice in biomedical research, control of crucial physiological parameters such as blood pressure and arterial blood gases is difficult to achieve in temporarily anesthetized mice due to lack of techniques for reversible arterial cannulation. Here we report that arterial blood pressure and blood gases can be measured reliably in anaesthetized and artificially ventilated mice using non-invasive technology.C57B16 mice were anaesthetized by i.p. injection of midazolam, fentanyl, and medetomidin, intubated, and ventilated for 3 h. End tidal pCO(2) was monitored by micro-capnometry. Arterial blood pressure was measured non-invasively using a tail cuff.Non-invasive blood pressure (NIBP) correlated strongly with the invasive arterial blood pressure measured at the external carotid artery (r = 0.99, P < 0.001) and end tidal PCO2 values correlated very well with arterial blood PCO2 (r= 0.93, P < 0.001). The current results demonstrate that it is possible to reliably measure and control the most relevant physiological parameters in anesthetized mice. Thereby the current study may help to reduce animal numbers and perform mice experiments under more defined and controlled physiological conditions in the future. (c) 2006 Elsevier B.V. All rights reserved.