Bio-Corrosion of Stainless Steel by Osteoclasts-In Vitro Evidence
Bio-Corrosion of Stainless Steel by Osteoclasts-In Vitro Evidence
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DOI：
10.1002/jor.20831
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发表时间：
2009-07-01
影响因子：
2.8
通讯作者：
Filgueira, Luis
中科院分区：
文献类型：
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作者：
Cadosch, Dieter;Chan, Erwin;Filgueira, Luis
大多数与生物系统接触的金属都会受到电化学过程的腐蚀。本研究探讨了人类破骨细胞（OC）是否能够在不锈钢（SS）上生长，并直接腐蚀金属合金，导致形成相应的金属离子，这可能会引起炎症反应并激活免疫系统。扫描电子显微镜分析表明，长期可行的OC文化和明显的吸收功能的表面上的SS光盘OC培养21天。原子发射光谱法研究证实了这一发现，显示OC培养物上清液中铬、镍和锰的水平显着增加。此外，通过细胞计数珠阵列分析，在相同的培养物中发现了显着水平的促炎细胞因子IL-1 β、IL-6和TNF-alpha，这些细胞因子被认为是骨质溶解的主要介质。在本研究中，它表明，人破骨细胞，前体细胞能够生长和分化为成熟的OC上SS。成熟细胞能够直接腐蚀金属表面并释放相应的金属离子，其诱导促炎细胞因子的分泌，所述促炎细胞因子已知增强破骨细胞分化、活化和存活。因此，增强的腐蚀和随后释放的金属离子可能导致假体周围骨中的溶骨性病变增强，从而导致植入物的无菌性松动。(C)2008骨科研究学会。由威利期刊公司出版J Orthop Res 27：841-846，2009
Most metals in contact with biological systems undergo corrosion by an etectrochemical process. This study investigated whether human osteoclasts (OC) are able to grow on stainless steel (SS) and directly corrode the metal alloy leading to the formation of corresponding metal ions, which may cause inflammatory reactions and activate the immune system. Scanning electron microscopy analysis demonstrated long-term viable OC cultures and evident resorption features on the surface of SS discs on which OC were cultured for 21 days. The findings were confirmed by atomic emission spectrometry investigations showing significantly increased levels of chromium, nickel, and manganese in the supernatant of OC cultures. Furthermore, significant levels of pro-inflammatory cytokines IL-1 beta, IL-6, and TNF-alpha, which are considered to be major mediators of osteolysis, were revealed in the same cultures by cytometric bead array analysis. Within the present study, it was shown that human osteoclast, precursors are able to grow and differentiate towards mature OC on SS. The mature cells are able to directly corrode the metal surface and release corresponding metal ions, which induce the secretion of pro-inflammatory cytokines that are known to enhance osteoclast differentiation, activation, and survival. Enhanced corrosion and the subsequently released metal ions may therefore result in enhanced osteolytic lesions in the peri-prosthetic bone, contributing to the aseptic loosening of the implant. (C) 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27:841-846, 2009