Antihepatocarcinoma Effect of Portulaca oleracea L. in Mice by PI3K/Akt/mTOR and Nrf2/HO-1/NF-B Pathway
Antihepatocarcinoma Effect of Portulaca oleracea L. in Mice by PI3K/Akt/mTOR and Nrf2/HO-1/NF-B Pathway
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马齿苋PI3K/Akt/mTOR和Nrf2/HO-1/NF-B通路对小鼠的抗肝癌作用
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发表时间：
2017
影响因子：
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通讯作者：
Ling Changquan
中科院分区：
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作者：
Zheng Guoyin;Peng Hao;Li Min;Gu Wei;Chen Zhe;Ling Changquan
本研究旨在评价马齿苋的药理作用。目的：研究马齿苋（PL）对N-亚硝基二乙胺（NDEA-）诱导的肝细胞癌（HCC）的抑制作用，并探讨其可能的作用机制。将小鼠随机分为对照组、NDEA组、NDEA +马齿苋（100 mg/kg）组和NDEA +马齿苋组。（200 mg/kg）组。各组动物饮用含NDEA（100 ppm）的水。1h后将马齿苋溶于PBS中，连续灌胃7天。结果表明，马齿苋能降低肝脏和血清中丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）的活性。马齿苋还能降低血清中白细胞介素-6（IL-6）、IL-1、肿瘤坏死因子-（TNF-）和甲烷二甲醛（MDA）的含量，恢复超氧脱氢酶（SOD）活性。马齿苋能明显减轻肝脏病理改变。此外，用马齿苋处理有效地抑制磷脂酰肌醇3激酶（PI 3 K）、蛋白激酶B（Akt）、哺乳动物雷帕霉素靶蛋白（mTOR）、核因子-κ B（NF-B）和NF-B抑制剂（IB）的磷酸化，并上调NF-E2相关因子2（Nrf 2）和血红素加氧酶-（HO-）1的表达。综上所述，我们的研究表明，马齿苋通过抗炎作用和通过PI 3 K/Akt/mTOR和Nrf 2/HO-1/NF-B通路对NDEA诱导的肝癌具有保护作用。antioxidative.properties
The purpose of the present study was to evaluate the pharmacological effects of Portulaca oleracea L. (Purslane) (PL) on.N-nitrosodiethylamine- (NDEA-) induced hepatocellular carcinomas (HCC) and explore its potential mechanism. Mice were.randomly assigned to four groups: control group, NDEA group, NDEA + Purslane (100mg/kg) group, and NDEA + Purslane.(200mg/kg) group. The animal of each group was given NDEA (100ppm) in drinking water. 1h later, Purslane dissolved in.PBS was intragastrically administered for continuous seven days. The results showed that Purslane reduced the activities of.alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in liver and serum. Purslane also reduced the contents.of interleukin-6 (IL-6), IL-1, tumor necrosis factor- (TNF-), and methane dicarboxylic aldehyde (MDA) and restored the.activity of superoxygen dehydrogenises (SOD) in serum. Purslane could obviously attenuate the hepatic pathological alteration..Furthermore, treatment with Purslane effectively inhibited the phosphorylations of phosphatidylinositol 3 kinase (PI3K), protein.kinase B (Akt), mammalian target of rapamycin (mTOR), nuclear factor-kappa B (NF-B), and inhibitor of NF-B (IB) and.upregulated the expressions of NF-E2-related factor 2 (Nrf2) and heme oxygenase- (HO-) 1. In conclusion, our research suggested.that Purslane exhibited protective effects on NDEA-induced hepatocellular carcinomas by anti-inflammatory and antioxidative.properties via the PI3K/Akt/mTOR and Nrf2/HO-1/NF-B pathway.