Interactions between syntaxins identify at least five SNARE complexes within the golgi/prevacuolar system of the arabidopsis cell
Interactions between syntaxins identify at least five SNARE complexes within the golgi/prevacuolar system of the arabidopsis cell
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DOI：
10.1091/mbc.12.12.3733
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发表时间：
2001-12-01
影响因子：
3.3
通讯作者：
Raikhel, NV
中科院分区：
文献类型：
--
作者：
Sanderfoot, AA;Kovaleva, V;Raikhel, NV
可溶性N-乙基马来酰亚胺敏感因子适配器蛋白受体(SNARES)的合成素家族在运输囊泡与特定细胞器的融合中发挥着重要作用。模式植物拟南芥的基因组中编码了24种合成素。这24个基因分属于10个基因家族，已被重新归类为植物合成素(SYPs)。其中一些基因家族的特征已经被发现，SYP2型合成素存在于泡前间隔(PVC)，SYP4型合成素存在于反式高尔基体网络(TGN)。在这里，我们报告了两个以前未被描述的合成素基团。SYP5组由两个成员的基因家族编码，而SYP61是单基因。这两种类型的合成素都定位于内膜系统的多个隔室，包括TGN和PVC。这两组合成素彼此形成圈套复合体，并与其他拟南芥形成圈套复合体。在TGN上，SYP61与SNARE VTI12以及SYP41或SYP42形成复合体。SYP51和SYP61相互作用，并与VTI12相互作用，很可能也在TGN上。在聚氯乙烯上，SYP5型合成素与SYP2型合成素以及SNARE VTI11特异地相互作用，形成可能参与TGN到PVC运输的SNAR复合体。
The syntaxin family of soluble N-ethyl maleimide sensitive factor adaptor protein receptors (SNAREs) is known to play an important role in the fusion of transport vesicles with specific organelles. Twenty-four syntaxins are encoded in the genome of the model plant Arabidopsis thaliana. These 24 genes are found in 10 gene families and have been reclassified as syntaxins of plants (SYPs). Some of these gene families have been previously characterized, with the SYP2-type syntaxins being found in the prevacuolar compartment (PVC) and the SYP4-type syntaxins on the trans-Golgi network (TGN). Here we report on two previously uncharacterized syntaxin groups. The SYP5 group is encoded by a two-member gene family, whereas SYP61 is a single gene. Both types of syntaxins are localized to multiple compartments of the endomembrane system, including the TGN and the PVC. These two groups of syntaxins form SNARE complexes with each other, and with other Arabidopsis SNAREs. On the TGN, SYP61 forms complexes with the SNARE VTI12 and either SYP41 or SYP42. SYP51 and SYP61 interact with each other and with VTI12, most likely also on the TGN. On the PVC, a SYP5-type syntaxin interacts specifically with a SYP2-type syntaxin, as well as the SNARE VTI11, forming a SNARE complex likely involved in TGN-to-PVC trafficking.