Yes-Associated Protein Expression in Head and Neck Squamous Cell Carcinoma Nodal Metastasis
Yes-Associated Protein Expression in Head and Neck Squamous Cell Carcinoma Nodal Metastasis
复制标题
头颈鳞状细胞癌淋巴结转移中 Yes 相关蛋白的表达
DOI：
10.1371/journal.pone.0027529
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发表时间：
2011-11-09
期刊：
影响因子：
3.7
通讯作者：
Bhowmick, Neil A.
中科院分区：
文献类型：
--
作者：
Ge, Lin;Smail, Matthew;Bhowmick, Neil A.
简介：yes相关蛋白（YAP）被认为是一种在多种肿瘤中扩增的癌基因，包括头颈部鳞状细胞癌（HNSCC）。然而，YAP表达在HNSCC中的作用尚不清楚。基于YAP在hippo通路中的核心作用，我们测试了YAP是否与HNSCC的进展阶段和转移潜力有关。方法：采用全组织和组织芯片免疫组化方法检测YAP在人良性和恶性鳞癌组织中的表达。YAP在微阵列组织中的表达首先与临床病理因素相关，结果在整个组织切片样本中得到验证。为了研究YAP和p63在调节HNSCC上皮向间质转化中的作用，我们在Fadu细胞和SCC-25细胞中检测了上皮和间质标志物，这些细胞是内源性YAP表达升高和sirna介导的表达下调的HNSCC细胞。结果：对人HNSCC组织的分析表明，与良性组织相比，YAP在肿瘤中的表达升高，并特异性地定位于肿瘤侵袭前（p值）
Introduction: Yes-associated protein (YAP) is considered an oncogene found amplified in multiple tumors, including head and neck squamous cell carcinoma (HNSCC). However, the role for YAP expression in HNSCC is not understood. Based on the central role of YAP in the hippo pathway, we tested if YAP was associated with the stage of HNSCC progression and metastatic potential.Methods: To determine the expression of YAP in human benign and HNSCC tissue specimens, immunohistochemical analyses were performed in whole tissue samples and tissue microarrays. The expression of YAP in tissues of microarray was first associated with clinic-pathologic factors and results verified in samples from whole tissue sections. To investigate the role of YAP and p63 in regulating HNSCC epithelial to mesenchymal transition, epithelial and mesenchymal markers were assayed in Fadu and SCC-25 cells, HNSCC cells with endogenously elevated YAP expression and siRNA-mediated expression knockdown.Results: Analysis of human HNSCC tissues suggested YAP expression was elevated in tumors compared to benign tissues and specifically localized at the tumor invasive front (p value