LAMININ PROMOTES ATTACHMENT AND NEURITE ELONGATION OF FETAL HYPOTHALAMIC NEURONS GROWN IN SERUM-FREE MEDIUM
LAMININ PROMOTES ATTACHMENT AND NEURITE ELONGATION OF FETAL HYPOTHALAMIC NEURONS GROWN IN SERUM-FREE MEDIUM
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DOI：
10.1016/0304-3940(84)90225-8
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发表时间：
1984-01-01
影响因子：
2.5
通讯作者：
TIXIERVIDAL, A
中科院分区：
文献类型：
--
作者：
FAIVREBAUMAN, A;PUYMIRAT, J;TIXIERVIDAL, A
层粘连蛋白作为涂层或溶液，可以使胎儿[小鼠]下丘脑细胞在无血清培养基中快速附着，并显着增强神经突网络发育。与在胎牛血清涂层上生长的培养物相比，细胞保持成簇并且星形胶质细胞变成纤维状。层粘连蛋白在非神经元细胞中通过免疫细胞化学可视化。在无血清培养基中生长的培养物中层粘连蛋白阳性细胞的数量低于在补充血清的培养基中生长的培养物。在两种培养条件下，它们的数量随着体外时间的推移而减少。
Laminin, as a coating or in solution, allows a rapid attachment of fetal [mouse] hypothalamic cells in serum-free medium, and strikingly enhances the neurite network development. As compared to cultures grown on a fetal calf serum coating, cells remain in clusters and astrocytes become fibrous. Laminin was visualized by immunocytochemistry in non-neuronal cells. The number of laminin-positive cells was lower in cultures grown in serum-free medium than in those grown in serum-supplemented medium. In both culture conditions, their number decreases with time in vitro.