A RIBOSOMAL DNA PHYLOGENY SUPPORTS THE CLOSE EVOLUTIONARY RELATIONSHIPS AMONG THE SPOROCHNALES, DESMARESTIALES, AND LAMINARIALES (PHAEOPHYCEAE) 1
A RIBOSOMAL DNA PHYLOGENY SUPPORTS THE CLOSE EVOLUTIONARY RELATIONSHIPS AMONG THE SPOROCHNALES, DESMARESTIALES, AND LAMINARIALES (PHAEOPHYCEAE) 1
复制标题
核糖体 DNA 系统发育支持孢子目、Desmarestiales 和昆布菌目（褐藻纲）之间密切的进化关系 1
DOI：
10.1111/j.0022-3646.1996.00112.x
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发表时间：
1996
影响因子：
2.9
通讯作者：
L. Druehl
中科院分区：
文献类型：
--
作者：
I. H. Tan;L. Druehl
我们报道了6个完整的18S核核体DNA （rDNA）序列，分别代表5个褐藻目：Sporochnus comosus C. A. Agardh （Sporochnales）、Chorda tomentosa Lyngbye （Chordaceae, Laminariales）、Saccorhiza polyschides (Lightfoot) Batters （Phyllariaceae, Laminariales）、Desmarestia ligulata (Lightfoot) Lamouroux （Desmarestiales）、Ectocarpus silulosus (Dillwyn) Lyngbye （Ectocarpales）和Scytosiphon lomentaria (Lyngbye) J. G. Agardh （Scytosiphonales）。将这些序列与已发表的层藻（Alaria marginata Postel et Ruprecht [Alariaceae]和Macrocystis integrifolia Bory [Lessoniaceae]）和fucalean (Fucus gardneri Silva) rDNA序列进行比较，利用距离矩阵和简化法进行系统发育推断。推断的18S系统发育系将Sporochnus、Desmarestia、Chorda、Saccorhiza、Alaria和Macrocystis聚集在一个组合中。这个孢子- desmarestiales - laminariales （S‐D‐L）复合体通过bootstrap分析从Ectocarpales， Scytosiphonales和Fucales中分离出来。推断的系统发生与导致S - D - L复合体的几种可能的进化过程相一致。这个组合的成员在精子中缺乏眼点，它们的精子具有非典型的褐藻鞭毛：前鞭毛短，后鞭毛长。此外，它们是同卵异型的，在微观配子体和宏观孢子体之间进行异型世代交替。S - D - L复合体的成员可以根据其减数孢子中眼点的存在与否来划分为不同的系统发育系。我们的发现支持了Sporochnales， Desmarestiales和Laminariales密切相关的论点。此外，我们的rDNA树表明，层压鱼是副食。
We report six complete 18S ribosomal DNA (rDNA) sequences representing five brown algal orders: Sporochnus comosus C. A. Agardh (Sporochnales), Chorda tomentosa Lyngbye (Chordaceae, Laminariales), Saccorhiza polyschides (Lightfoot) Batters (Phyllariaceae, Laminariales), Desmarestia ligulata (Lightfoot) Lamouroux (Desmarestiales), Ectocarpus siliculosus (Dillwyn) Lyngbye (Ectocarpales), and Scytosiphon lomentaria (Lyngbye) J. G. Agardh (Scytosiphonales). These sequences were compared with published laminarialean (Alaria marginata Postel et Ruprecht [Alariaceae] and Macrocystis integrifolia Bory [Lessoniaceae]) and fucalean (Fucus gardneri Silva) rDNA sequences for phylogeny inference using both the distance‐matrix and parsimony methods. The inferred 18S phylogenies clustered Sporochnus, Desmarestia, Chorda, Saccorhiza, Alaria, and Macrocystis in an assemblage. This Sporochnales–Desmarestiales–Laminariales (S‐D‐L) complex was consistently separated from the Ectocarpales, Scytosiphonales, and Fucales by bootstrap analyses. The inferred phylogenies are consistent with several possible evolutionary processes leading to this S‐D‐L complex. Members in this assemblage lack eyespots in their sperm, and their sperm have the atypical brown algal flagellation: shorter anterior and longer posterior flagella. In addition, they are oogamous with a heteromorphic alternation of generations between a microscopic gametophyte and a macroscopic sporophyte. Members of the S‐D‐L complex can be separated into different phylogenetic lines based on the presence/absence of eyespots in their meiospores. Our findings support the contention that the Sporochnales, Desmarestiales, and Laminariales are closely related. In addition, our rDNA tree suggests that the Laminariales is paraphyletic.