Extraction of plasma from whole blood using a deposited microbead plug (DMBP) in a capillary-driven microfluidic device
Extraction of plasma from whole blood using a deposited microbead plug (DMBP) in a capillary-driven microfluidic device
复制标题
使用毛细管驱动微流体装置中的沉积微珠塞 (DMBP) 从全血中提取血浆
DOI：
10.1007/s10544-012-9635-x
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发表时间：
2012-06-01
影响因子：
2.8
通讯作者：
Li, Jingmin
中科院分区：
文献类型：
--
作者：
Li, Chunyu;Liu, Chong;Li, Jingmin
我们提出了一种基于沉积微珠塞（DMBP）的微流控装置，能够通过毛细作用力从全血中提取血浆。该器件是通过将具有直通道的PDMS板可逆地结合到亲水性玻璃基板来制造的。通过微珠的自然沉积方法，在2 min内容易地在通道的入口处构建DMBP，而不需要堰或光聚合。主要在亲水性玻璃基板上产生的毛细管力在DMBP和等离子体提取的制造期间提供驱动力，从而简化操作。DMBP只允许血浆通过，但阻止血细胞，这是用绵羊血实验证明的。DMBP能够在血浆提取期间保持其初始配置。获得了高质量的血浆，没有微珠和血细胞的污染。这种易于使用、易于集成、一次性的基于DMBP的微流体装置具有与芯片上生物分析单元集成的潜力，用于即时诊断的应用。
We presented a deposited microbead plug (DMBP)-based microfluidic device capable of extracting plasma from whole blood by capillary forces. This device was fabricated by reversibly bonding a PDMS slab with a straight channel to a hydrophilic glass substrate. The DMBP was easily constructed at the inlet of the channel within 2 min by a method of natural deposition of microbeads without the need of weirs or photopolymerization. Capillary forces generated mainly on the hydrophilic glass substrate provided a driving force during the fabrication of the DMBP and plasma extraction, resulting in simplicity of operations. The DMBP only allows blood plasma to pass through but blocks blood cells, which was demonstrated experimentally using sheep blood. The DMBP enabled to remain in its initial configuration during plasma extraction. The high quality plasma was obtained without contamination of microbeads and blood cells. This easy-to-use, easy-to-integrate, disposable the DMBP-based microfluidic device has the potential to be integrated with on-chip bioanalytical units for the applications of point-of-care diagnostics.