Use of nested PCR for differential diagnosis of falcipargm malaria reinfection and relapse in drug-resistant patients
Use of nested PCR for differential diagnosis of falcipargm malaria reinfection and relapse in drug-resistant patients
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DOI：
10.1023/a:1021968517124
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发表时间：
2002-10-01
影响因子：
0.7
通讯作者：
Rabinovich, SA
中科院分区：
文献类型：
--
作者：
Van Ha, N;Dao, LD;Rabinovich, SA
显微镜检查不能区分耐药恶性疟原虫感染复发和再感染。然而，这种鉴别诊断对于适当的治疗是必不可少的。以3个高度多态性的恶性疟原虫基因（msp1、msp2和glurp）及其反映这些基因结构状态的等位基因为遗传标记，利用内部引物进行PCR鉴别诊断。在27名患者中，这些等位基因的特征在用artersunate治疗前和治疗结束后14-28天反复出现症状期间是相同的，这证明疟疾复发。在24例患者中，甲氟喹治疗前和2-3个月后反复出现症状时，这些等位基因的结构不同，证明存在再感染。
Microscopic examination does not allow differentiation of drug-resistant P. falciparum infection relapse from reinfection. However, this differential diagnosis is essential for adequate therapy. Three highly polymorphic P. falciparum genes (msp1, msp2, and glurp) and their alleles reflecting the structural state of these genes were used as genetic markers for differential diagnosis by PCR with internal primers. In 27 patients the characteristics of these alleles were identical before treatment with artersunate and during repeated manifestation of symptoms 14-28 days after the end of therapy, which attested to malaria relapses. In 24 patients the structure of these allele before mefloquine therapy and during repeated manifestation of the symptoms after 2-3 months was different, which attested to reinfection.