Antiproliferation and apoptosis induced by tamoxifen in human bile duct carcinoma QBC939 cells via upregulated p53 expression
Antiproliferation and apoptosis induced by tamoxifen in human bile duct carcinoma QBC939 cells via upregulated p53 expression
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DOI：
10.1016/j.bbrc.2009.05.059
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发表时间：
2009-07-24
影响因子：
3.1
通讯作者：
Chen, Qing-Xi
中科院分区：
文献类型：
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作者：
Han, Peng;Kang, Jin-He;Chen, Qing-Xi
他莫昔芬 (TAM) 是一种非甾体抗雌激素，用于治疗乳腺癌已有 30 多年的历史。最近的研究表明，TAM 对肝癌、胰腺癌等胃肠道肿瘤也具有疗效，TAM 的化学预防活性可能是由于其抑制细胞生长和诱导细胞凋亡的能力。在本研究中，我们采用MTT法、倒置显微镜、荧光显微镜、透射电镜、经典DNA片段化琼脂糖凝胶电泳法、PI单染和FITC/PI双染流式细胞术以及Western blotting等方法，研究了他莫昔芬对人胆管癌（BDC细胞系QBC939）生长和凋亡的影响。我们的数据显示，TAM可以显着抑制QBC939细胞的生长并诱导细胞凋亡。在 TAM 处理的细胞中观察到 p53，表明 p53 可能在 TAM 诱导的 QBC939 细胞凋亡中发挥重要作用。这些结果为了解 TAM 对 BDC 的抗癌作用提供了重要的见解。 (C) 2009 Elsevier Inc. 保留所有权利。
Tamoxifen (TAM) is a nonsteroidal antiestrogen that has been used in the treatment of breast cancer for over 30 years. Recently, it was shown that TAM also has efficacy on gastrointestinal neoplasms such as hepatocarcinoma and pancreatic carcinoma, and that the chemopreventive activities of TAM might be due to its abilities to inhibit cell growth and induce apoptosis. In the present study, we investigated the effects of tamoxifen on growth and apoptosis in the human bile duct carcinoma (BDC cell line QBC939 using MTT assay, inverted microscopy, fluorescence microscopy, transmission election microscopy. classic DNA fragmentation agarose gel electrophoresis assay, PI single- and FITC/PI double-staining flow cytometry, and Western blotting. Our data revealed that TAM could significantly inhibit growth and induce apoptosis in QBC939 cells. Increased expression of p53 was observed in TAM-treated cells, indicating that p53 might play an important role in TAM-induced apoptosis in QBC939 cells. These results provide significant insight into the anticarcinogenic action of TAM on BDC. (C) 2009 Elsevier Inc. All rights reserved.