Isolation of purple nonsulfur bacteria from the digestive tract of ayu (Plecoglossus altivelis).
Isolation of purple nonsulfur bacteria from the digestive tract of ayu (Plecoglossus altivelis).
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从香鱼（Plecoglossus altivelis）消化道中分离紫色非硫细菌。
DOI：
10.1093/bbb/zbac001
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Hitoshi Miyasaka
中科院分区：
文献类型：
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作者：
Aoi Koga;Takumi Yamasaki;Shuhei Hayashi;Shinjiro Yamamoto;Hitoshi Miyasaka
据报道，紫色非硫细菌(PNSB)在鱼类中具有益生菌作用，但它们是否存在于鱼类的消化道中仍是一个需要回答的问题。本研究从日本熊本市熊本市的久马河(Plecoglossus Altivelis)鱼的消化道中分离到12株PNSB。所有分离的PNSB都属于红假单胞菌属。从库马河沿岸的土壤样品中也分离到5株红假单胞菌。根据pufLM基因部分序列构建的系统发育树表明，来自阿育的PNSB与土壤中的PNSB相似。还比较了生长介质中的氯化钠浓度对阿育PNSB和土壤中PNSB生长的影响。在较高的氯化钠浓度下，Ayu的PNSB表现出较好的生长性能，这表明Ayu的肠道可能为嗜盐微生物提供了合适的环境。
Purple nonsulfur bacteria (PNSB) reportedly have probiotic effects in fish, but whether they are indigenous in the digestive tract of fish is a question that requires answering. We attempted to isolate PNSB from the digestive tract of ayu (Plecoglossus altivelis) from the Kuma River (Kumamoto, Japan) and successfully isolated 12 PNSB strains. All the isolated PNSB belonged to the genusRhodopseudomonas. FiveRhodopseudomonasstrains were also isolated from the soil samples collected along the Kuma River. The phylogenetic tree based on the partial sequence ofpufLMgene indicated that the PNSB from ayu and soil were similar. The effects of NaCl concentration in growth medium on growth were also compared between the PNSB from ayu and soil. The PNSB from ayu showed a better growth performance at a higher NaCl concentration, suggesting that the intestinal tract of ayu, a euryhaline fish, might provide suitable environment for halophilic microorganisms.