Fentanyl Assay Derived from Intermolecular Interaction-Enabled Small Molecule Recognition (iMSR) with Differential Impedance Analysis for Point-of-Care Testing
Fentanyl Assay Derived from Intermolecular Interaction-Enabled Small Molecule Recognition (iMSR) with Differential Impedance Analysis for Point-of-Care Testing
复制标题
源自分子间相互作用的小分子识别 (iMSR) 的芬太尼测定以及用于即时测试的差分阻抗分析
DOI：
10.1021/acs.analchem.2c00017
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发表时间：
2022
影响因子：
7.4
通讯作者：
Dong, Pei
中科院分区：
文献类型：
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作者：
Wang, Zhe;Nautiyal, Amit;Alexopoulos, Christopher;Aqrawi, Rania;Huang, Xiaozhou;Ali, Ashraf;Lawson, Katherine E.;Riley, Kevin;Adamczyk, Andrew J.;Dong, Pei
快速有效地区分和定量体液中的小分子药物（例如芬太尼）是诊断和个人用药的主要挑战。然而，目前用于测量药物浓度和代谢物的毒理学方法需要基于实验室的测试，这不是及时治疗患者的有效或具有成本效益的方法。在这里，我们展示了一种通过将分子间相互作用支持的小分子识别（iMSR）与共轭聚合物的差分阻抗分析相结合来监测芬太尼水平的测定方法。与设计的锚界面的不同相互作用是通过柔性导电聚合物的电状态的扰动来转换的。该检测显示芬太尼对常见干扰以及通过测试条或皮肤贴片在可变体液中具有出色的选择性。直接使用患者血液，与“金”标准方法 LC-MS 结果相比，传感器提供的平均偏差为 1%–5%，医学相关的芬太尼范围为 20–90 nM。卓越的传感特性与机械灵活性和兼容性相结合，实现了即时检测，并为生物标志物检测范围之外的应用提供了一条有前景的途径。
Rapid and effective differentiation and quantification of a small molecule drug, such as fentanyl, in bodily fluids are major challenges for diagnosis and personal medication. However, the current toxicology methods used to measure drug concentration and metabolites require laboratory-based testing, which is not an efficient or cost-effective way to treat patients in a timely manner. Here, we show an assay for monitoring fentanyl levels by combining the intermolecular interaction-enabled small molecule recognition (iMSR) with differential impedance analysis of conjugated polymers. The differential interactions with the designed anchor interface were transduced through the perturbance of the electric status of the flexible conducting polymer. This assay showed excellent fentanyl selectivity against common interferences, as well as in variable body fluids through either testing strips or skin patches. Directly using the patient blood, the sensor provided 1%–5% of the average deviation compared to the “gold” standard method LC-MS results in the medically relevant fentanyl range of 20–90 nM. The superior sensing properties, in conjunction with mechanical flexibility and compatibility, enabled point-of-care detection and provided a promising avenue for applications beyond the scope of biomarker detection.