Occurrence of canine parvovirus type 2c in the United States
Occurrence of canine parvovirus type 2c in the United States
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DOI：
10.1177/104063870701900512
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发表时间：
2007-09-01
影响因子：
1.5
通讯作者：
Saliki, Jeremiah T.
中科院分区：
文献类型：
--
作者：
Hong, Charles;Decaro, Nicola;Saliki, Jeremiah T.
犬细小病毒2型(CPV-2)于1978年左右出现，是世界范围内引起犬只死亡的主要病原。在1980年代中期，最初的CPV-2已经进化，并被两个变种完全取代，即CPV-2a和CPV-2b。2000年，在意大利发现了一种新的CPV变种(名为CPV-2c)，目前在该国与2a和2b型共同传播。越南和西班牙的单次暴发也报告了CPV-2c。这项研究是为了确定CPV-2c是否在美国发生。2006年4月至2007年4月期间，从16个州的狗身上采集了33份粪便样本，并使用实时聚合酶链式反应(PCR)对其进行了CPV检测。阳性样本进一步用常规聚合酶链式反应和小槽结合TaqMan聚合酶链式反应检测，以确定病毒类型并区分疫苗毒株和野毒株。27个样本呈CPV阳性，其中7个样本是来自亚利桑那州、加利福尼亚州、佐治亚州、俄克拉何马州和德克萨斯州的CPV-2c。在这7个分离株中，有4个分离株与欧洲分离株不同，在4076和4104位增加了2个单核苷酸突变，后者在靠近主要抗原位点的残基440处产生ThrAla突变。检测到CPV-2c的州从东海岸到西海岸的地理分布强烈表明，这种新的CPV变种可能在美国广泛存在。CPV的不断进化要求基于单抗和基于核酸的诊断方法应定期检查对流行的CPV毒株的敏感性。
Canine parvovirus (CPV) type 2 (CPV-2) emerged around 1978 as a major pathogen of dogs worldwide. In the mid-1980s, the original CPV-2 had evolved and was completely replaced by 2 variants, CPV-2a and CPV-2b. In 2000, a new variant of CPV (named CPV-2c) was detected in Italy and now cocirculates with types 2a and 2b in that country. The CPV-2c has also been reported from single outbreaks in Vietnam and Spain. This study was conducted to determine if CPV-2c occurs in the United States. Thirty-three fecal samples were collected from dogs in 16 states between April 2006 and April 2007 and were tested for CPV using real-time polymerase chain reaction (PCR). Positive samples were further tested using conventional PCR and minor-groove binding TaqMan PCR assays to determine the viral type and to differentiate vaccine strains from field strains. Twenty-seven samples were positive for CPV, 7 of which were CPV-2c from 5 states: Arizona, California, Georgia, Oklahoma, and Texas. Of the 7 isolates, 4 differed from European CPV-2c isolates by 2 additional single-nucleotide mutations at positions 4076 and 4104, the latter of which produces a ThrAla change at residue 440 located near a major antigenic site. The coast-to-coast geographic distribution of the states in which CPV-2c was detected strongly suggests that this new CPV variant is probably widespread in the United States. The continuous evolution of CPV requires that monoclonal antibody-based and nucleic acid-based diagnostic assays should be periodically checked for sensitivity on prevalent CPV strains.