Prickle promotes neurite outgrowth via the Dishevelled dependent pathway in C1300 cells
Prickle promotes neurite outgrowth via the Dishevelled dependent pathway in C1300 cells
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DOI：
10.1016/j.neulet.2009.09.050
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发表时间：
2009-12-18
影响因子：
2.5
通讯作者：
Hatano, Masahiko
中科院分区：
文献类型：
--
作者：
Fujimura, Lisa;Watanabe-Takano, Haruko;Hatano, Masahiko
鼠皮刺和皮刺2属于平面细胞极性基因。维甲酸（RA）诱导C1300神经母细胞瘤细胞在神经突样过程形成过程中诱导了Prickle2基因的表达，而非Prickle1基因的表达。在没有RA的情况下，C1300细胞中过表达prickl或Prickle2可诱导惊人的神经突样过程形成。在果蝇中，由于Prickle与dishevelle （Dvl）结合诱导其降解，我们研究了Dvl蛋白在C1300细胞神经突样过程形成中的参与。在诱导神经突样突起形成后，Dvl1蛋白的数量减少。在C1300细胞中，prickl和Prickle2与Dvl1有关联，过表达的结果是Dvl1蛋白的减少。此外，在C1300细胞中，Dvl1的过表达阻止了prickl或Prickle2过表达诱导的神经突样过程的形成。因此，prickl和Prickle2通过依赖于Dvl1的机制促进C1300细胞神经突样过程的形成。2009爱思唯尔爱尔兰有限公司版权所有。
Murine Pricklel and Prickle2 belong to the planar cell polarity genes. Prickle2 but not Prickle1 gene expression was induced in C1300 neuroblastoma cells during neurite-like process formation induced by retinoic acid (RA). Over-expression of Pricklel or Prickle2 in C1300 cells induced striking neurite-like process formation in the absence of RA. Since Prickle binds to Dishevelled (Dvl) to induce its degradation in Drosophila, we examined the participation of Dvl protein in the neurite-like process formation of C1300 cells. Upon induction of the neurite-like process formation, the amount of Dvl1 protein decreased. Pricklel and Prickle2 could associate with Dvl1 and over-expression of Pricklel or Prickle2 resulted in the reduction of Dvl1 protein in C1300 cells. Furthermore, over-expression of Dvl1 in C1300 cells prevented the neurite-like process formation induced by Pricklel or Prickle2 over-expression. Thus, Pricklel and Prickle2 promote neurite-like process formation of C1300 cells via the Dvl1 dependent mechanism. (C) 2009 Elsevier Ireland Ltd. All rights reserved.