Various Molecular Species of Diacylglycerol Hydroperoxide Activate Human Neutrophils via PKC Activation.
Various Molecular Species of Diacylglycerol Hydroperoxide Activate Human Neutrophils via PKC Activation.
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DOI：
10.3164/jcbn.2007009
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发表时间：
2007-07
影响因子：
2.4
通讯作者：
Yamamoto Y
中科院分区：
文献类型：
--
作者：
Kambayashi Y;Takekoshi S;Tanino Y;Watanabe K;Nakano M;Hitomi Y;Takigawa T;Ogino K;Yamamoto Y
我们提出了双甘油过氧化氢诱导的非调控信号转导导致氧化应激相关疾病。在这项研究中，我们调查了过氧化氢双甘油的哪些分子物种激活了人外周中性粒细胞。在本研究中测试的所有双甘油过氧化氢、双甘油氢氧化物和双甘油均可诱导中性粒细胞产生超氧化物。在含有相同脂肪酸组成的分子物种中，激活中性粒细胞的能力如下：二酰基甘油氢过氧化氢>；二酰基甘油氢氧化物≥二酰甘油。亚油酸组成的双甘油过氧化氢对中性粒细胞的激活作用强于花生四烯酸组成的双甘油。1-棕榈酰基-2-亚油酰甘油过氧化氢(PLG-OHO)对中性粒细胞的刺激作用最强。我们再次证实了中性粒细胞中存在PLG-OOH激活的蛋白激酶C(PKC)。PLG-OHO可诱导中性粒细胞p47Phox的磷酸化，N-甲酰甲硫基亮氨酰-苯丙氨酸或4-β-佛波醇-12β-肉豆蔻酸-13α-乙酸酯也能诱导中性粒细胞p47Phox的磷酸化。此外，p47Phox磷酸化的时间进程与超氧化物产生的时间进程相当。这些结果表明，通过不受控制的过程产生的PLG-OOH激活了细胞内蛋白激酶C。PLG-OHO可以作为生物第二信使导致氧化应激引起的炎症性疾病。
We have proposed that diacylglycerol hydroperoxide-induced unregulated signal transduction causes oxidative stress-related diseases. In this study, we investigated which molecular species of diacylglycerol hydroperoxide activated human peripheral neutrophils. All diacylglycerol hydroperoxides, diacylglycerol hydroxides, and diacyglycerols tested in the present study induced superoxide production by neutrophils. The ability to activate neutrophils among molecular species containing the same fatty acid composition was as follows; diacylglycerol hydroperoxide>diacylglycerol hydroxide≥diacylglycerol. The diacylglycerol hydroperoxide composed of linoleate was a stronger activator for neutrophils than that composed of arachidonate. 1-Palmitoyl-2-linoleoylglycerol hydroperoxide (PLG-OOH) was the strongest stimulator for neutrophils. We reconfirmed that PLG-OOH activated protein kinase C (PKC) in neutrophils. PLG-OOH induced the phosphorylation of p47phox, a substrate of PKC and a cytosolic component of NADPH oxidase, in neutrophils, as did N-formyl-methionyl-leucyl-phenylalanine or 4β-phorbol-12β-myristate-13α-acetate. Moreover, the time course of p47phox phosphorylation was comparable to that of superoxide production. These results suggest that PLG-OOH activated intracellular protein kinase C. PLG-OOH, produced via an uncontrolled process, can act as a biological second messenger to cause inflammatory disease from oxidative stress.
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影响因子：
3.9
作者：
Churchill, EN;Szweda, LI
通讯作者：
Szweda, LI
DOI：
10.1161/01.atv.0000012782.59850.41
发表时间：
2002-04-01
影响因子：
8.7
作者：
Huber, J;Boechzelt, H;Leitinger, N
通讯作者：
Leitinger, N
DOI：
10.1016/j.bbalip.2005.08.011
发表时间：
2005-10-01
影响因子：
4.8
作者：
Matsura, T;Togawa, A;Yamada, K
通讯作者：
Yamada, K
DOI：
10.1073/pnas.86.17.6758
发表时间：
1989-09-01
影响因子：
11.1
作者：
GOPALAKRISHNA, R;ANDERSON, WB
通讯作者：
ANDERSON, WB
影响因子：
64.8
作者：
Bochkov, VN;Kadl, A;Leitinger, N
通讯作者：
Leitinger, N