Evaluation of Backcrossed Pyramiding Lines of the Yield-related Gene and the Bacterial Leaf Blight Resistant Genes
Evaluation of Backcrossed Pyramiding Lines of the Yield-related Gene and the Bacterial Leaf Blight Resistant Genes
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发表时间：
2020
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通讯作者：
Shuto Yamada;Yusuke Kurokawa;Keisuke Nagai;Rosalyn B. Angeles‐Shim;H. Yasui;N. Furuya;A. Yoshimura
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作者：
Shuto Yamada;Yusuke Kurokawa;Keisuke Nagai;Rosalyn B. Angeles‐Shim;H. Yasui;N. Furuya;A. Yoshimura
产量相关基因 WFP 通过增加水稻每穗的初级分枝数量来增加籽粒数量。在本研究中，WFP从ST-12基因渗入到IRBB60，IRBB60是一个金字塔系，具有IR24遗传背景下的BLB抗性基因Xa4、xa5、xa13和Xa21。金字塔品系 PL-1、PL-4 和 PL-5 包含在基于其改进的 PBN 的初始 BC2F2 选择中，被选择用于进一步的农艺评估和世代进展直至 BC2F3。由于 WFP 基因渗入，所有三个品系都记录了显着较高的 PBN 和 GN，尽管在育种过程中至少丢失两个 BLB 基因导致了这些品系对不同 BLB 种族的不同反应。在这些品系中，只有 PL-5 与 IRBB60 相比，以每平方米穗重衡量的实际产量估计值显着更高。 PL-5 还对用于抗性筛选的 6 个 BLB 种族中的 5 个表现出抗性。尽管对籽粒大小有负面影响，但 PL-5 产量预估的提高以及经过验证的 BLB 抗性使其成为东南亚热带水稻生态系统下采用的合适品种。
The yield-related gene, WFP increases grain number by increasing the primary branch number per panicle in rice. In the present study, WFP was introgressed from ST-12 to IRBB60, a pyramiding line having the genes Xa4, xa5, xa13 and Xa21 for BLB resistance in the IR24 genetic background. The pyramided lines PL-1, PL-4 and PL-5 that were included in the initial BC2F2 selections based on their improved PBN were selected for further agronomic evaluation and generation advance up to BC2F3. All three lines recorded significantly higher PBN and GN due WFP introgression, although the loss of at least two BLB genes during the breeding process resulted in the variable response of the lines to the different BLB races. Among the lines, only PL-5 showed a significantly higher estimate of actual yield measured in terms of panicle weight per square meter compared to IRBB60. PL-5 also exhibited resistance to five out of the six BLB races used for resistance screening. Despite the negative effects in grain size, the improved yield estimates, as well as the validated BLB resistance of PL-5 makes it a suitable candidate for cultivar adoption under the tropical rice ecosystem of Southeast Asia.