Ethanol modulates spontaneous calcium waves in axonal growth cones in vitro.
Ethanol modulates spontaneous calcium waves in axonal growth cones in vitro.
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DOI：
10.3390/brainsci3020615
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发表时间：
2013-04-23
期刊：
影响因子：
3.3
通讯作者：
Mazurkiewicz JE
中科院分区：
文献类型：
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作者：
Lindsley TA;Mazurkiewicz JE
在发育中的神经元的频率长时间，自发，短暂的钙离子（Ca2+）升高本地化的生长锥，是成反比的轴突伸长的速度和增加几倍时，轴突暂停。在这里，我们报告说，这些自发的Ca2+瞬变与缓慢的动力学，称为Ca2+波，乙醇暴露的条件下，改变轴突生长动力学调制。使用时间序列荧光钙成像，我们发现，急性治疗胎鼠海马神经元与43或87 mM乙醇在培养的早期发展阶段的轴突生长锥显示至少一个Ca2+波在10分钟的记录，从18%的控制到5%的文化暴露于乙醇的百分比下降。慢性暴露于43 mM乙醇也降低了Ca2+波的发病率为8%，但暴露于87 mM乙醇增加其发病率为31%。慢性或急性乙醇均不影响Ca2+波的峰值振幅、达峰时间或总持续时间。在一些实验中，我们确定了时间之间的相关性Ca2+波和轴突生长动力学的生长和非生长阶段。正如预期的那样，在所有治疗组中，波在静止或收缩的生长锥中最普遍，除了长期暴露于87 mM乙醇的培养物。因此，生长锥Ca 2+波和轴突生长动力学之间的关系被乙醇破坏。
In developing neurons the frequency of long duration, spontaneous, transient calcium (Ca2+) elevations localized to the growth cone, is inversely related to the rate of axon elongation and increases several fold when axons pause. Here we report that these spontaneous Ca2+ transients with slow kinetics, called Ca2+ waves, are modulated by conditions of ethanol exposure that alter axonal growth dynamics. Using time-series fluorescence calcium imaging we found that acute treatment of fetal rat hippocampal neurons with 43 or 87 mM ethanol at an early stage of development in culture decreased the percent of axon growth cones showing at least one Ca2+ wave during 10 min of recording, from 18% in controls to 5% in cultures exposed to ethanol. Chronic exposure to 43 mM ethanol also reduced the incidence of Ca2+ waves to 8%, but exposure to 87 mM ethanol increased their incidence to 31%. Neither chronic nor acute ethanol affected the peak amplitude, time to peak or total duration of Ca2+ waves. In some experiments, we determined the temporal correlation between Ca2+ waves and growth and non-growth phases of axonal growth dynamics. As expected, waves were most prevalent in stationary or retracting growth cones in all treatment groups, except in cultures exposed chronically to 87 mM ethanol. Thus, the relationship between growth cone Ca2+ waves and axon growth dynamics is disrupted by ethanol.