Synaptonemal complex protein SYCP3 of the rat: Evolutionarily conserved domains and the assembly of higher order structures
Synaptonemal complex protein SYCP3 of the rat: Evolutionarily conserved domains and the assembly of higher order structures
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DOI：
10.1159/000102105
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发表时间：
2007-01-01
影响因子：
2.3
通讯作者：
Benavente, R.
中科院分区：
文献类型：
--
作者：
Baier, A.;Alsheimer, M.;Benavente, R.
SYCP3 是脊椎动物联会复合体的主要结构蛋白成分，也是雄性生育力的重要决定因素，至少在哺乳动物中如此。 SYCP3聚合特性的阐明将为我们理解联会复合体在减数分裂前期如何组装和分解提供重要信息。为此，我们研究了不同 SYCP3 结构域对高阶结构组装的可能贡献。我们观察到分子的进化保守结构域（即α螺旋以及两个侧翼基序CM1和CM2）对于SYCP3聚合不仅是必要的，而且也是充分的。最近的研究强调了这些结果与生殖生物学的相关性，这些研究表明，α-螺旋和 CM2 末端的缺失会导致人类减数分裂破坏和不育。版权所有 (C) 2007 S. Karger AG，巴塞尔。
SYCP3 is a major structural protein component of vertebrate synaptonemal complexes as well as an important determinant of male fertility, at least in mammals. The elucidation of SYCP3 polymerization properties would provide important information towards our understanding as to how synaptonemal complexes are assembled and disassembled during meiotic prophase. To this end we have investigated the possible contribution of different SYCP3 domains to the assembly of higher order structures. We observed that the evolutionarily conserved domains of the molecule (i.e. the alpha-helix together with the two flanking motifs CM1 and CM2) are not only necessary but also sufficient for SYCP3 polymerization. The relevance of these results for reproduction biology is underscored by recent studies showing that the deletion of the very end of the alpha-helix and CM2 leads to meiosis disruption and infertility in humans. Copyright (C) 2007 S. Karger AG, Basel.